FastQCFastQC Report
Wed 31 May 2023
EGAF00006172666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38386
Sequences flagged as poor quality0
Sequence length200
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA1873048.79383108424947No Hit
GTTTTAATTTTTGTAGAGAAGGAGTCTTGCTATGTTGCCCAGGCTGGTCT15464.027510029698327No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA10452.7223466888969936No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA9562.4904913249622256No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA9132.3784713176678998No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA8922.323763872245089No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA7091.8470275621320273No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCTTAAAGAGTGTTTTAGATCA5231.3624759026728495No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA3861.0055749492002293No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA3520.9170009899442505No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTA2660.6929609753555984No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTACAGAGTGTTTTAGATCA2630.6851456260094826No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2600.6773302766633669No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA2520.6564893450737248No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2090.5444693377793988No Hit
CACGATTAACCCAAGTCAATAGAAGCCTGCGTAAAGAGTGTTTTAGATCA2010.5236284061897567No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTTTTTTAGATCA1760.45850049497212525No Hit
TACAGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGAGTA1730.4506851456260095No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAGAGAGTGTTTTAGATCA1590.41421351534413586No Hit
CACGATTAACCCTAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA1470.38295211795967277No Hit
CACGATTAACCCAAGTCAATATAAGCCGGCGTAAAGAGTGTTTTAGATCA1460.38034700151096756No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAATAGTGTTTTAGATCA1380.3595060699213255No Hit
CACGATTAACCCAAGTCCATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA1270.33084978898556766No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTCAAGAGTGTTTTAGATCA1210.3152190902933361No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1210.3152190902933361No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1100.2865628093575783No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA1070.2787474600114625No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1060.27614234356275724No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGCGCGTA1050.273537227114052No Hit
TACGGAAGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1050.273537227114052No Hit
TACGAAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGTGTGTA1040.2709321106653467No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1030.2683269942166415No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA990.2579065284218205No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA950.24748606262699943No Hit
TACGAAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGAGTGTA930.2422758297295889No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAGGAGTGTTTTAGATCA920.23967071328088366No Hit
AACTAACGATCGCCTACTGGGGAGCAGAGTTTCTACCCAGAAGAGGAAAC890.23185536393476786No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA830.21622466524253633No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAACGAGTGTTTTAGATCA730.19017350075548378No Hit
CACGATTAACCCAAGTCAATAGAAACCGGCATAAAGAGTGTTTTAGATCA700.182358151409368No Hit
CACGATTAACCCAAGTCAATAGAAGCCTGCTTAAAGAGTGTTTTAGATCA690.17975303496066275No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA640.16672745271713646No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATTA640.16672745271713646No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA630.1641223362684312No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATCATTGGGCGTAAAGAGGGAGCA620.16151721981972594No Hit
CACGATTAACCCAAGTCGATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA620.16151721981972594No Hit
TACGGAGGGTGCGAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA620.16151721981972594No Hit
CACGATTAACCCAAGTCAATAGAAACCGGCGTAAAGAGTGTTTTAGATCA620.16151721981972594No Hit
CACGATTAACCCAAGTCAATAGACGCCGGCGTAAAGAGTGTTTTAGATCA610.15891210337102069No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGCGTGTTTTAGATCA590.15370187047361017No Hit
TACGTAGGATGCAAGCGTTATCCGGAATGACTGGGCGTAAAGGGTGCGTA590.15370187047361017No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA580.1510967540249049No Hit
TACGGAGGGAGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTA580.1510967540249049No Hit
CACGATTAACCCAAGTCAATAGAGGCCGGCGTAAAGAGTGTTTTAGATCA560.1458865211274944No Hit
TACGTAGGGAGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA550.14328140467878914No Hit
CGCGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA530.13807117178137862No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCTCGTA520.13546605533267336No Hit
TACGTAGGGTGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGCTCGTA500.13025582243526285No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA480.12504558953785233No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGCAAAGAGTGTTTTAGATCA470.1224404730891471No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCG470.1224404730891471No Hit
GTTTTAATTTTTGTAGAGAAGTAGTCTTGCTATGTTGCCCAGGCTGGTCT470.1224404730891471No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGGGTGTTTTAGATCA460.11983535664044183No Hit
CATGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA460.11983535664044183No Hit
CACGATTAGCCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA450.11723024019173658No Hit
CACGATTCACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA440.11462512374303131No Hit
CACGATTAACCCAAGCCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA420.1094148908456208No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGACCA420.1094148908456208No Hit
GTTTTAATTTTTGTAGCGAAGGAGTCTTGCTATGTTGCCCAGGCTGGTCT410.10680977439691554No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGGTCA400.10420465794821028No Hit
GACAGAGGGTGCGAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA400.10420465794821028No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGCTTTAGATCA400.10420465794821028No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTCTTAGATCA390.10159954149950502No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA390.10159954149950502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)