FastQCFastQC Report
Wed 31 May 2023
EGAF00006172667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38386
Sequences flagged as poor quality0
Sequence length200
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT1674243.61485958422342No Hit
CAGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAAAAGGCAGAGCAGA10242.6676392434741834No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA9442.4592299275777627No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA8312.1648517688740685No Hit
CCCAGTTTGTGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT8222.1414057208357216No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA6691.7428229041838172No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGTGTAGCC6241.6255926639920804No Hit
CCCAGTTTGGGTCTTAGCTCTTGTGTGTTCAGATATGTTAAAGCCACTTT4171.0863335591100922No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATCTGTTAAAGCCACTTT3760.9795237847131768No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCATATATGTTAAAGCCACTTT3610.9404470379825978No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG3510.9143958734955453No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG3470.9039754077007242No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA3170.8258219142395665No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGCTATGTTAAAGCCACTTT2910.7580888865732298No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA2570.669514927317251No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGGTCCAGTT2560.6669098108685458No Hit
CCCAGTTTTGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT2550.6643046944198405No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA2550.6643046944198405No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCCCTTT2320.6043870160996196No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA2210.5757307351638619No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATCCGTCCAGAT1720.4480800291773042No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1170.3047986244985151No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1140.2969832751523993No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA1140.2969832751523993No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1100.2865628093575783No Hit
CAGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAACAGGCAGAGCAGA1090.283957692908873No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG1040.2709321106653467No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGCATCCCAGGG990.2579065284218205No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA980.2553014119731152No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA970.2526962955244099No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTATATGGCCAGAT940.2448809461782942No Hit
CCTGTTTGATCCCCATGCTTTCGAGCCTCAGCGTCAATATACAGCCAGAT900.23446048038347317No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTAATGGCCCAGAG880.22925024748606262No Hit
CCCATTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT880.22925024748606262No Hit
CAGGTAGTTAGACCAGCACGTAAAGCGTCTAATACAAAAGGCAGAGCAGA870.22664513103735737No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG810.21101443234512585No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA790.2058041994477153No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTTTTCAGATATGTTAAAGCCACTTT770.20059396655030481No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA730.19017350075548378No Hit
CCCAGTTTGTGTCTTAGCTCTTGTGTGTTCAGATATGTTAAAGCCACTTT700.182358151409368No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA690.17975303496066275No Hit
CCCAGTTTGGGTCTTAGTTATTCTGTGTTCAGATGCATTAAAGCCACTTT680.1771479185119575No Hit
CCCAGTTTGTGTCTTAGCTATTGTGTGTTCAGATCTGTTAAAGCCACTTT680.1771479185119575No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA670.17454280206325223No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG660.17193768561454698No Hit
CCACTACAGGGCTTGGGGCAAATTTTAAAAGGTACCCTTCCCGTGGGTTA660.17193768561454698No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA650.16933256916584172No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA650.16933256916584172No Hit
CCCAGTTTGGTTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT640.16672745271713646No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC640.16672745271713646No Hit
CCCAGTTTGTGTCTTAGCTATTGTGTGTTCATATATGTTAAAGCCACTTT630.1641223362684312No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA590.15370187047361017No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTGCCCCAGGG590.15370187047361017No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACGGCCCAGTA580.1510967540249049No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAGTATCGTATTAGTA570.14849163757619965No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCAGTCCAGTG560.1458865211274944No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG550.14328140467878914No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG540.14067628823008388No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG540.14067628823008388No Hit
CCCAGTTTGGGTCTTAGCTCTTGTGTGTTCAGATCTGTTAAAGCCACTTT520.13546605533267336No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAACGGGCCAGTG520.13546605533267336No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACCTT510.1328609388839681No Hit
CCCAGTTTGGGTCTTAGCTCTTGTGTGTTCAGATATGTTAAAGCCCCTTT510.1328609388839681No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA500.13025582243526285No Hit
CAGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAAAAGGCCGAGCAGA500.13025582243526285No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA490.1276507059865576No Hit
CCGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAAAAGGCAGAGCAGA480.12504558953785233No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGCTCAGATATGTTAAAGCCACTTT480.12504558953785233No Hit
CCCAGTTTGGGTCTTAGCTATCGTGTGTTCAGATATGTTAAAGCCACTTT480.12504558953785233No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA480.12504558953785233No Hit
CCCAGTTTGGGTCTTAGCTATTGTGCGTTCAGATATGTTAAAGCCACTTT470.1224404730891471No Hit
CCCAGTTTGGGCCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT450.11723024019173658No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGCTAAAGCCACTTT450.11723024019173658No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGCGTAGTA450.11723024019173658No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA450.11723024019173658No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGACCCAGTG450.11723024019173658No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGGCCAGAG440.11462512374303131No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAGGCCACTTT440.11462512374303131No Hit
CCCAGTTTGTGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCCCTTT430.11202000729432605No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCGGATATGTTAAAGCCACTTT430.11202000729432605No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTCT420.1094148908456208No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACCGTCCAGAT410.10680977439691554No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA410.10680977439691554No Hit
CCCAGTTTGGGTCTTAGCTATTGCGTGTTCAGATATGTTAAAGCCACTTT400.10420465794821028No Hit
CCCAGTTTGTGTCTTAGCTATTGTGTGTTCAGCTATGTTAAAGCCACTTT390.10159954149950502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)