FastQCFastQC Report
Sun 21 May 2023
EGAF00006172704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40510
Sequences flagged as poor quality0
Sequence length200
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA570314.078005430757838No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA442310.918291779807456No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATCATTGGGCGTAAAGAGGGAGCA37239.190323376943963No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA25436.277462354974081No Hit
GTTTTAATTTTTGTAGAGAAGGAGTCTTGCTATGTTGCCCAGGCTGGTCT24245.983707726487287No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA23295.749197728955814No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA22425.53443594174278No Hit
TACGTAGGGCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGTA16574.090348062206862No Hit
TACGTATGGAGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGTACGCA8692.1451493458405335No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA6141.5156751419402616No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA5971.473710195013577No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCTCGTA4961.2243890397432733No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATCATTGGGCGTAAAGCGGGAGCA4711.1626758824981487No Hit
TACGTAGGGGGCTAGCGTTGTCCGGAATCACTGGGCGTAAAGGGTTCGCA4661.1503332510491235No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA4131.0195013576894594No Hit
TACGTAAGGTCCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGTACGTA3730.92076030609726No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA3200.7899284127375956No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA2600.6418168353492965No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA2470.6097259935818317No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA2340.5776351518143669No Hit
TACGTATGGACCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGTACGTA2160.5332016785978771No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGTTCGCA1970.4862996790915823No Hit
TACGTAAGGGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGTGCGTA1870.46161441619353244No Hit
TACGTAGGGGGCGAGCGTTATCCGGAATCATTGGGCGTAAAGCGCGCGCA1810.4468032584547026No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1620.3999012589484078No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA1410.3480622068625031No Hit
TACGATTAACCCAAGCTAATAAAACTCGGCATAAAGAGTGTTTAAGGTCT1230.30362873364601334No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA1190.2937546284867934No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA1130.2789434707479635No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCTTAAAGAGTGTTTTAGATCA1090.2690693655887435No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTA1060.26166378671932855No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA960.23697852382127868No Hit
TACGTATGGAGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGTCCGCA960.23697852382127868No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA910.22463589237225376No Hit
CACGATTAACCCAAGTCAATAGAAACCGGCATAAAGAGTGTTTTAGATCA890.2196988397926438No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGAGTTTAAAGGGAGCGTA830.20488768205381386No Hit
GTTTTAATTTTTGTAGCGAAGGAGTCTTGCTATGTTGCCCAGGCTGGTCT760.18760799802517897No Hit
AACTAACGATCGCCTACTGGGGAGCAGAGTTTCTACCCAGAAGAGGAAAC760.18760799802517897No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATCATTGAGCGTAAAGAGGGAGCA660.1629227351271291No Hit
TACGTAGGTGGCGAGCGTTATCCAGAATCATTGGGCGTAAAGAGGGAGCA620.15304862996790916No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA600.1481115773882992No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA590.1456430510984942No Hit
CACGATTAACCCAAGTCAATAGAAACCGGCGTAAAGAGTGTTTTAGATCA570.1407059985188842No Hit
GTTTTAATTTTTGTAGAGAAGTAGTCTTGCTATGTTGCCCAGGCTGGTCT550.13576894593927427No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTACAGAGTGTTTTAGATCA530.13083189335966428No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA520.1283633670698593No Hit
GTTTTAATTTTTGTCGAGAAGGAGTCTTGCTATGTTGCCCAGGCTGGTCT510.1258948407800543No Hit
GTTTTAATTTTTGTAGAGAAGGAGTCTTGCTCTGTTGCCCAGGCTGGTCT500.12342631449024932No Hit
TACGTAGGGGGGCTAGCGTTGTCCGGAATCACTGGGCGTAAAGGGTTCGC490.12095778820044434No Hit
CACGATTAACCCAAGTCAATAGAAGCCTGCGTAAAGAGTGTTTTAGATCA470.11602073562083436No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA450.1110836830412244No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA450.1110836830412244No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGAGCGTAAAGGGCTCGTA420.10367810417180943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)