FastQCFastQC Report
Wed 31 May 2023
EGAF00006172705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40510
Sequences flagged as poor quality0
Sequence length200
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG531513.120217230313502No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT40229.928412737595655No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC35018.642310540607259No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA26076.4354480375216No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG25276.2379659343372005No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG15963.939767958528758No Hit
CAGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAAAAGGCAGAGCAGA15573.843495433226364No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA14713.631202172303135No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA9262.2858553443594176No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTTTGTGTCCAGAA8592.1204640829424837No Hit
CCTATTTGCTCCCCACGCTTTCGGGCATGAGCGTCAGTTGCGGCCCAGAC6621.6341644038509011No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA5871.449024932115527No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA4671.1528017773389287No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG4471.103431251542829No Hit
CCTGTTTGCTACCCACGCTTTCGTTCCTCAGCGTCAGAATGAGCCCAGTA4391.083683041224389No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGAG3400.8392989385336953No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTATGGCCAGAA2320.5726980992347568No Hit
CCTGTTTGCTACCCACGCTTTCGTTCCTCAGCGTCAGTTGAAGTCCAGCA2170.535670204887682No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGATGATGACCAGAA2080.5134534682794372No Hit
CAGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAACAGGCAGAGCAGA1990.4912367316711923No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTCAAGTCCAGAA1990.4912367316711923No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA1920.47395704764255736No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA1610.3974327326586028No Hit
CAGGTAGTTAGACCAGCACGTAAAGCGTCTAATACAAAAGGCAGAGCAGA1600.3949642063687978No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1580.39002715378918784No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGATGCCGGCCCAGGA1580.39002715378918784No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGAG1400.34559368057269807No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1360.33571957541347813No Hit
CCCAGTTTGTGCCTTAGCTGTTGTGTCCTCAGGACATTAAAGTCCCTTTC1360.33571957541347813No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA1240.3060972599358183No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA1200.2962231547765984No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGTCGGACCAGTT1170.28881757590718343No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1080.2666008392989386No Hit
CCTGTTTGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTTGTGTCCAGAA1040.2567267341397186No Hit
CCCAGTTTTGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT1020.2517896815601086No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCATATATGTTAAAGCCACTTT1010.24932115527030366No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCTTTAGCGTCAATACCGGTCCAGAT970.2394470501110837No Hit
CCCAGTTTGTGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT920.22710441866205874No Hit
CCCAGTTTGGGTCTTAGTTATTCTGTGTTCAGATGCATTAAAGCCACTTT900.2221673660824488No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGGTTATGGCCAGAA880.21723031350283883No Hit
CCTGTTCGATACCCTCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG850.20982473463342383No Hit
CCCAGTTTGGGTCTTAGCTCTTGTGTGTTCAGATATGTTAAAGCCACTTT840.20735620834361887No Hit
CAGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAAAAGGCCGAGCAGA830.20488768205381386No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA820.2024191557640089No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG770.19007652431498395No Hit
CCGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAAAAGGCAGAGCAGA750.18513947173537398No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATCTGTTAAAGCCACTTT750.18513947173537398No Hit
CCTGTTTGCTACCCACGCTTTCGTTCCTCAGCGTCAGCATGAGCCCAGTA650.1604542088373241No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA620.15304862996790916No Hit
GATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC600.1481115773882992No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCCCTTT560.13823747222907923No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGCTATGTTAAAGCCACTTT550.13576894593927427No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAGCGTCAGTTATGCTACAGTA540.1333004196494693No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG540.1333004196494693No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATGCGTTAAAGCCACTTT520.1283633670698593No Hit
AATGATACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTG500.12342631449024932No Hit
AATGATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTG470.11602073562083436No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTA450.1110836830412244No Hit
GATACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTC440.10861515675141942No Hit
CCACTACAGGGCTTGGGTCAAATTTTAAAAGGTACCCTTCCCGTGGGTTA440.10861515675141942No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA420.10367810417180943No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG420.10367810417180943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)