FastQCFastQC Report
Sun 21 May 2023
EGAF00006172710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153321
Sequences flagged as poor quality0
Sequence length200
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA9085959.26063618160591No Hit
GTTTTAATTTTTGTAGAGAAGGAGTCTTGCTATGTTGCCCAGGCTGGTCT1747011.39439476653557No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCTTAAAGAGTGTTTTAGATCA25531.6651339346860508No Hit
AACTAACGATCGCCTACTGGGGAGCAGAGTTTCTACCCAGAAGAGGAAAC13820.9013768498770552No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTACAGAGTGTTTTAGATCA12170.7937594980465821No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA10920.7122312012053144No Hit
CACGATTAACCCAAGTCAATAGAAGCCTGCGTAAAGAGTGTTTTAGATCA9310.6072227548737615No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA9120.5948304537538889No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTTTTTTAGATCA8380.5465657020238585No Hit
CACGATTAACCCTAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA7830.5106932514137007No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAGAGAGTGTTTTAGATCA7340.47873415905192374No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA7000.4565584623110989No Hit
CACGATTAACCCAAGTCAATATAAGCCGGCGTAAAGAGTGTTTTAGATCA6670.4350349919450043No Hit
CACGATTAACCCAAGTCCATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA6600.4304694073218933No Hit
CACGATTAACCCAAGTCAATAGAAACCGGCATAAAGAGTGTTTTAGATCA6000.39133582483808477No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAATAGTGTTTTAGATCA5760.3756823918445614No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTCAAGAGTGTTTTAGATCA5660.36916012809726No Hit
GTTTTAATTTTTGTAGCGAAGGAGTCTTGCTATGTTGCCCAGGCTGGTCT5620.36655122259833944No Hit
CACGATTAACCCAAGTCAATAGAAACCGGCGTAAAGAGTGTTTTAGATCA4040.2634994553909771No Hit
GTTTTAATTTTTGTAGAGAAGTAGTCTTGCTATGTTGCCCAGGCTGGTCT3990.2602383235173264No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGCGTGTTTTAGATCA3840.25045492789637425No Hit
GTTTTAATTTTTGTCGAGAAGGAGTCTTGCTATGTTGCCCAGGCTGGTCT3640.23741040040177142No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA3610.23545372127758102No Hit
GTTTTAATTTTTGTAGAGAAGGAGTCTTGCTCTGTTGCCCAGGCTGGTCT3500.22827923115554946No Hit
GTTTTAATTTTTGTAGAGAAGGAGTCTTGCTATATTGCCCAGGCTGGTCT3240.21132134541256578No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATTA3190.20806021353891507No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAGGAGTGTTTTAGATCA3180.20740798716418493No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA3170.2067557607894548No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAACGAGTGTTTTAGATCA3030.1976245915432328No Hit
CACGATTAACCCAAGTCAATAGACGCCGGCGTAAAGAGTGTTTTAGATCA2970.193711233294852No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGTGCGTA2800.18262338492443958No Hit
TTCGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2770.18066670580024916No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTA2630.17153553655402717No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGATTGTTTTAGATCA2500.16305659368253533No Hit
TACGATTAACCCAAGCTAATAAAACTCGGCATAAAGAGTGTTTAAGGTCT2460.16044768818361477No Hit
CACGATTAACCCAAGTCAATAGAAGCCTGCTTAAAGAGTGTTTTAGATCA2320.15131651893739279No Hit
CACGATTCACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA2270.14805538706374208No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA2230.1454464815648215No Hit
TACGGAGGGTGCGAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA2100.13696753869332967No Hit
CACGATTAACCCAAGTCGATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA2040.13305418044494882No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCG1940.1265319166976474No Hit
TTCCAGCTCCAATAACGTATATTAAAGTTGCTGCACTTAAAAAGCTCGTA1890.12327078482399671No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGGTCA1830.11935742657561586No Hit
CACGATTAACCCAAGTCAGTAGAAGCCGGCGTAAAGAGTGTTTTAGATCA1790.1167485210766953No Hit
CGCGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA1780.11609629470196514No Hit
CACGATTAACCCAAGTTAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA1760.11479184195250487No Hit
CACGATTAACCCAAGTCAATAGAGGCCGGCGTAAAGAGTGTTTTAGATCA1710.11153071007885416No Hit
CACGATTAACCCAAGTCAATAGGAGCCGGCGTAAAGAGTGTTTTAGATCA1710.11153071007885416No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1680.10957403095466374No Hit
CACGATTAGCCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA1680.10957403095466374No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGGGTGTTTTAGATCA1620.10566067270628289No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA1570.1023995408326322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)