FastQCFastQC Report
Wed 31 May 2023
EGAF00006172749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153519
Sequences flagged as poor quality0
Sequence length200
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTTAGCA1963412.789296438877273No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA117317.641399435900443No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA76935.011106117158136No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG74554.856076446563618No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA56963.7102899315394184No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG54723.5643796533328125No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA51133.3305323770999027No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA48913.185924869234427No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA47983.1253460483718625No Hit
CCCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA38192.4876399663885254No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG31772.0694506868856624No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG30992.0186426435815763No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA29901.9476416599899686No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA28481.8551449657697092No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG28351.8466769585523617No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA25661.671454347670321No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT22951.49492896644715No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA20851.3581380806284564No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA20201.3157980445417181No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA12690.8266077814472476No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTGACACTCCCGTG11620.7569095681967705No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA11100.7230375393273797No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA10230.6663670294882067No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA9460.6162103713546857No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA8480.5523746246392955No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGGTCGTCCAGTA8080.5263192178166872No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT7400.48202502621825305No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGTA7300.47551117451260105No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTTTGTGTCCAGAA7180.4676945524658186No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA6310.41102404262664555No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA5950.3875741764862981No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG5930.3862714061451677No Hit
CCTGTTTGCTACCCATGCTTTCGTGCTTCAGCGTCAGTTAAAGCCCAGTA5800.37780339892782No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA5550.36151876966368984No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCCTAGCA5460.35565630312860297No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTACAGCTTAGCA5460.35565630312860297No Hit
CAGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAAAAGGCAGAGCAGA5270.34327998488786404No Hit
CCCGTTTGCTACCCTGGCTTTCGCCTCTCAGCGTCAGACACAGTCCAGAA5190.3380689035233424No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGGCCAGGC4970.32373842977090783No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA4860.31657319289469055No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT4850.31592180772412537No Hit
CCCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGCCACAGTCCAGAA4750.30940795601847326No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGGCGGCCCAGAA4610.3002885636305604No Hit
CCTGTTTGATCCCCATGCTTTCGAGCCTCAGCGTCAATATACAGCCAGAT4560.29703163777773434No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA4230.27553592714908254No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA3990.25990268305551756No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA3940.2566457572026915No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA3780.24622359447364822No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGATCGGCGGCGGCA3610.2351500465740397No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA3480.226682039356692No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA3390.22081957282160514No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGATGATGACCAGAA3270.21300295077482265No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTG3180.20714048423973577No Hit
CCTGTTTGCTACCCACGCTTTCGTTCCTCAGCGTCAGTTGAAGTCCAGCA3180.20714048423973577No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA3020.1967183215106925No Hit
CCTGTTTGCTACCCACGCTTTCGTTCCTCAGCGTCAGAATGAGCCCAGTA2710.17652538122317107No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA2490.16219490747073653No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA2150.1400478116715195No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC1990.12962564894247616No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGCTATAGTTTGGTA1690.11008409382551998No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTCAAGTCCAGAA1690.11008409382551998No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTATAGCCCAGTA1670.10878132348438956No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCTGTTTCACCCCAGTA1560.10161608660817227No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG1540.10031331626704186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)