FastQCFastQC Report
Sun 21 May 2023
EGAF00006172756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69166
Sequences flagged as poor quality0
Sequence length200
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA3133645.30549692045224No Hit
GTTTTAATTTTTGTAGAGAAGGAGTCTTGCTATGTTGCCCAGGCTGGTCT878712.7042188358442No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA19372.8005089205679092No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA15032.1730329930890897No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA13701.9807419830552584No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCTTAAAGAGTGTTTTAGATCA8921.2896509845878033No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA7811.1291675100482896No Hit
AACTAACGATCGCCTACTGGGGAGCAGAGTTTCTACCCAGAAGAGGAAAC6710.9701298325766996No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA6070.8775988202295925No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA5530.799525778561721No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA5500.7951883873579504No Hit
TACGTATGGACCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGTACGTA4610.6665124483127548No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTACAGAGTGTTTTAGATCA3650.5277159297920944No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA3540.5118121620449354No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA3400.4915710030940057No Hit
CACGATTAACCCAAGTCAATAGAAGCCTGCGTAAAGAGTGTTTTAGATCA3110.4496428881242229No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA3010.43518491744498744No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTTTTTTAGATCA3010.43518491744498744No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA2920.4221727438336755No Hit
CACGATTAACCCAAGTCCATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA2700.39036520833935745No Hit
GTTTTAATTTTTGTAGCGAAGGAGTCTTGCTATGTTGCCCAGGCTGGTCT2690.3889194112714339No Hit
TACGATTAACCCAAGCTAATAAAACTCGGCATAAAGAGTGTTTAAGGTCT2550.36867825232050433No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA2490.360003469912963No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAGAGAGTGTTTTAGATCA2450.35422028164126884No Hit
CACGATTAACCCTAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA2440.35277448457334526No Hit
CACGATTAACCCAAGTCAATATAAGCCGGCGTAAAGAGTGTTTTAGATCA2260.32675013735072145No Hit
GTTTTAATTTTTGTAGAGAAGTAGTCTTGCTATGTTGCCCAGGCTGGTCT2160.31229216667148596No Hit
CACGATTAACCCAAGTCAATAGAAACCGGCGTAAAGAGTGTTTTAGATCA2120.3065089783997918No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAATAGTGTTTTAGATCA2030.2934968047884799No Hit
CACGATTAACCCAAGTCAATAGAAACCGGCATAAAGAGTGTTTTAGATCA2020.29205100772055637No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA1980.28626781944886215No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTCAAGAGTGTTTTAGATCA1910.27614723997339735No Hit
GTTTTAATTTTTGTAGAGAAGGAGTCTTGCTCTGTTGCCCAGGCTGGTCT1700.24578550154700285No Hit
GTTTTAATTTTTGTCGAGAAGGAGTCTTGCTATGTTGCCCAGGCTGGTCT1660.24000231327530866No Hit
GTTTTAATTTTTGTAGAGAAGGAGTCTTGCTATATTGCCCAGGCTGGTCT1600.23132753086776742No Hit
TACGTAGGGTCCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1540.22265274846022615No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA1460.21108637191683774No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAGGAGTGTTTTAGATCA1370.19807419830552586No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1270.18361622762629037No Hit
TACGAAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGTGTGTA1230.17783303935459618No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA1150.1662666628112078No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGCGTGTTTTAGATCA1120.16192927160743717No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA1080.15614608333574298No Hit
TACGTAAGGACCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGTACGTA1040.15036289506404882No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA1010.14602550386027818No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAACGAGTGTTTTAGATCA1000.14457970679235463No Hit
TACGGAGGGTGCGAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA950.13735072145273688No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATTA950.13735072145273688No Hit
CACGATTCACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA940.13590492438481336No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA940.13590492438481336No Hit
CACGATTAACCCAAGTCAATAGACGCCGGCGTAAAGAGTGTTTTAGATCA930.1344591273168898No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA880.12723014197727206No Hit
TTTTGTATTTTTAGTAGAGATGGGGTTTCTCCATGTTGGTCAGGCTGGTC850.12289275077350142No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTCATTGGGCGTAAAGAGCGCGTA790.11421796836596015No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGGGTGTTTTAGATCA750.10843478009426596No Hit
CACGATTAACCCAAGTCAATAGAAGCCTGCTTAAAGAGTGTTTTAGATCA750.10843478009426596No Hit
CACGATTAACCCGAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA730.10554318595841887No Hit
CACGATTAACCCAAGTCAATAGAGGCCGGCGTAAAGAGTGTTTTAGATCA720.10409738889049533No Hit
CGCGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA720.10409738889049533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)