FastQCFastQC Report
Sun 21 May 2023
EGAF00006172770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124639
Sequences flagged as poor quality0
Sequence length200
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA2424719.453782523929107No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1601512.849108224552507No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1426711.44665794815427No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA79026.3399096590954676No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA75606.065517213713203No Hit
GTTTTAATTTTTGTAGAGAAGGAGTCTTGCTATGTTGCCCAGGCTGGTCT53724.310047416940123No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA52834.238641195773393No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA37022.9701778736992432No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA21331.7113423567262254No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA18431.4786704001155337No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA15811.2684633220741501No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGAGTA11860.9515480708285529No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA10800.8665024591018863No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA9500.7622012371729555No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA9040.7252946509519492No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA7900.6338305024911945No Hit
TACGTAGGTGGCAAACGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA7860.6306212341241506No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCTTAAAGAGTGTTTTAGATCA7360.5905053795361003No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA6740.5407617198469179No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA6260.5022504994423896No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA5100.40918171679811294No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA4920.3947400091464148No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA4170.33456622726433943No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3650.2928457384927671No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTACAGAGTGTTTTAGATCA3410.273590128290503No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3280.2631600060976099No Hit
TACGTAGGTGACAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGTA3140.2519275668129558No Hit
CACGATTAACCCAAGTCAATAGAAGCCTGCGTAAAGAGTGTTTTAGATCA2810.22545110278484262No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGAGTGTAAAGGGAGCGTA2420.19416073620616342No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGAGTTTAAAGGGAGCGTA2380.1909514678391194No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTTTTTTAGATCA2310.18533524819679234No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA2070.1660796379945282No Hit
CACGATTAACCCTAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA2070.1660796379945282No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2050.1644750038110062No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAGAGAGTGTTTTAGATCA1990.15966110126044014No Hit
AACTAACGATCGCCTACTGGGGAGCAGAGTTTCTACCCAGAAGAGGAAAC1950.15645183289339612No Hit
CACGATTAACCCAAGTCCATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA1900.15244024743459111No Hit
GTTTTAATTTTTGTAGCGAAGGAGTCTTGCTATGTTGCCCAGGCTGGTCT1850.14842866197578608No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1790.14361475942522003No Hit
CACGATTAACCCAAGTCAATATAAGCCGGCGTAAAGAGTGTTTTAGATCA1710.137196222691132No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGAGTGTAAAGGGAGCGCA1570.1259637834064779No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTCAAGAGTGTTTTAGATCA1560.1251614663147169No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAATAGTGTTTTAGATCA1560.1251614663147169No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1400.11232439284654082No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA1330.10670817320421377No Hit
GTTTTAATTTTTGTAGAGAAGTAGTCTTGCTATGTTGCCCAGGCTGGTCT1310.10510353902069176No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1270.10189427065364774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)