FastQCFastQC Report
Wed 31 May 2023
EGAF00006172779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275508
Sequences flagged as poor quality0
Sequence length200
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA16840313.202818014469528No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA964697.563182669179652No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA609124.775509052079642No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA596774.678684884767481No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG532854.177551218808507No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTTTGTGTCCAGAA406863.189787911953512No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT393733.086848534074267No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA337062.642554966334982No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG268142.1022212326382896No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACAGCCCAGAG247361.939305751120338No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTGACACTCCCGTG237661.8632576197091668No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGAG204701.604850773182136No Hit
CCTGTTTGCTACCCACGCTTTCGTTCCTCAGCGTCAGTTGAAGTCCAGCA203051.5919147508286893No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTGAGCGTCAGTATGTATCCAGAC194251.5229226316103075No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA131711.0326081843469426No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA120600.9455056338337353No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA116480.9132047780178565No Hit
CCTGTTTGATACCCGCACCTTCGAGCATCAGCGTCAGTCGCACTGCAGCA106090.8317470372588804No Hit
CCTGTTTGCTACCCACGCTTTCGTTCCTCAGCGTCAGAATGAGCCCAGTA104990.8231230223565826No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA101500.7957613750756561No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTCAAGTCCAGAA99410.7793757467612904No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG97160.7617357162793177No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG85240.6682827547926002No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA84050.6589531386710236No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC79260.6213994737782907No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGATAATGGCCAGAA72840.571066586803062No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG71570.5611097695976819No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGTGTCAGTAACAGCCCAGAG70930.5560921609272541No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGAG69730.546684144670202No Hit
CCTGTTTGCTCCCCACGCTTTCGTTCCTCAGCGTCAGATTGTGCCCAGCA69240.5428425380319057No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG68610.5379033294969534No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGACATACCTTTGTG67770.5313177181170169No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG64750.5076408772034359No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA63180.49533205593379265No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA62760.4920392502438244No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG60270.4725176165104413No Hit
CCTGTTTGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTTGTGTCCAGAA56580.4435879665200061No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA52970.41528551761337446No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTTAAGTCCAGTA49690.38957027317743204No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA47980.3761638500111328No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA46600.3653446313155229No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA44960.3524870090975517No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA44340.3476262006980748No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA42990.33704218240889117No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGATGATGACCAGAA41270.3235573591071165No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGAAGAAGTCTGGTA41080.3220677565330833No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG39840.31234613973412945No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG38950.3053685276768158No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG38910.30505492713491406No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTAGCGCGCTGGAA32990.2586420469334571No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGTGTCAGTTGCTGTCCAGAA32760.25683884381752214No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATCAGTGTCAGTTAGGTTCTAGCA32650.2559764423272924No Hit
CAGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAAAAGGCAGAGCAGA32340.2535460381275539No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA31640.2480580286442735No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA30690.24061001577410723No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG30510.23919881333554943No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCAGCCAGAA29260.2293987964011202No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA29050.22775239355613605No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA28700.22500838881449586No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGCTGCGGCCCAGAG27490.21552197242196833No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA27390.214737971067214No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTA27330.2142675702543614No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG27080.21230756686747557No Hit
CCTGTTCGATACCCGCACCTTCGAGCCTCAACGTCAGTAGGGACGCGGCA26920.21105316469986862No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGATATACCTTTGTG26660.2090147611775073No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCTCAGACCAGAA26450.2073683583325232No Hit
CCTGTTTGCTCCCCACGCTTTCGTCCCTGAGCGTCAGTATGTATCCAGAC25920.20321315115232522No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA25670.20125314776543937No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTATGGCCAGAA25660.2011747476299639No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGATTATGGCCAGAA25210.19764674153356937No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGTGTCAGTAATGGCCCAGAG24180.18957152757959966No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG23900.18737632378628752No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGACCAGGA23010.18039871172897387No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG22000.17248029804595502No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA21270.16675708815624835No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAGTCGTCCAGTA20760.16275868124700119No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTGTAATGTAGTA19750.15484026756398236No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTGAGCGTCAGTATGTCTCCAGAC18150.1422962458879129No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA17600.13798423843676402No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTAATGGCCAGAA16620.13030102516017147No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTTCGTCCAGAA15930.12489141581236653No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA15910.12473461554141564No Hit
CCTGTTTGCTACCCACGCTTTCGTTCCTCAGCGTCAGCATGAGCCCAGTA15670.12285301229000524No Hit
CCCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA15040.11791380375505288No Hit
CCTATTTGCTCCCCACGCTTTCGGGCATGAGCGTCAGTTGCGGCCCAGAC14880.11665940158744595No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA14870.1165810014519705No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA14680.11509139887793726No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTACCCAGAG14650.11485619847151096No Hit
CCCAGTTTGTGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT14240.11164179291701815No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGTGTCAGTAACGGCCCAGAG13910.10905458844632883No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA13690.10732978546586927No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG13560.10631058370468864No Hit
CCCAGTTTTGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT13000.10192017611806432No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAG12760.10003857286665391No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)