FastQCFastQC Report
Wed 31 May 2023
EGAF00006172798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220438
Sequences flagged as poor quality0
Sequence length200
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA3037413.77893103729847No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA2462611.171395131510902No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2270310.29904100019053No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA169087.6701839065859785No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA102574.653009009335959No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA92244.184396519656321No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA61272.7794663352053637No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA51772.3485061559259295No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA48952.2205790290240337No Hit
GTTTTAATTTTTGTAGAGAAGGAGTCTTGCTATGTTGCCCAGGCTGGTCT47642.1611518885128698No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA37371.6952612525971023No Hit
TACGTAGGATGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA35221.5977281593917563No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA31361.4226222339161125No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA29991.3604732396410781No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA19630.8904998230795054No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA17590.7979567951079215No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA15880.7203839628376233No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA15280.6931654251989222No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA14280.6478011958010869No Hit
TACGTAGGCGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA12340.5597945907692866No Hit
TACGTAGGGGGCTAGCGTTGTCCGGAATCACTGGGCGTAAAGGGTTCGCA10850.4922018889665121No Hit
TACGTATGGAGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGTACGCA9210.4178045527540624No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA9170.415989983578149No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA8720.3955760803491231No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA8190.37153303876827043No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA7580.343860858835591No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCTTAAAGAGTGTTTTAGATCA7220.3275297362523703No Hit
CCCCATTAGAAACCCCAGTAGTCCGGCTGACTGACTGAACTTAGGCCGAT6360.288516498970232No Hit
TACGTAAGGGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGTGCGTA6040.27399994556292473No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA5870.26628802656529277No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA5750.2608443190375525No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA5750.2608443190375525No Hit
CCCCATTAGAAACCCCTGTAGTCCGGCTGACTGACTGAACTTAGGCCGAT5390.2445131964543318No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA5240.23770856204465654No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGAGTTTAAAGGGAGCGTA4910.22273836634337094No Hit
CCCCATTAGAAACCCCGGTAGTCCGGCTGACTGACTGAACTTAGGCCGAT4830.21910922799154411No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGTTCGCA4790.2172946588156307No Hit
CCCCATTAGATACCCCAGTAGTCCGGCTGACTGACTGAACTTAGGCCGAT4720.21411916275778223No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA4660.21139730899391213No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA4650.21094366669993378No Hit
CCCCATTAGAAACCCTAGTAGTCCGGCTGACTGACTGAACTTAGGCCGAT3920.17782777923951407No Hit
AACTAACGATCGCCTACTGGGGAGCAGAGTTTCTACCCAGAAGAGGAAAC3670.16648672189005526No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGAGTGTAAAGGGAGCGTA3650.16557943730209856No Hit
CCCCATTAGAAACCCTTGTAGTCCGGCTGACTGACTGAACTTAGGCCGAT3620.1642185104201635No Hit
TACGTATGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA3590.16285758353822843No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3570.16195029895027171No Hit
CCCCATTAGATACCCCTGTAGTCCGGCTGACTGACTGAACTTAGGCCGAT3570.16195029895027171No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGTA3550.16104301436231502No Hit
CCCCATTAGAAACCCTGGTAGTCCGGCTGACTGACTGAACTTAGGCCGAT3350.151970168482748No Hit
CCCCATTAGATACCCCGGTAGTCCGGCTGACTGACTGAACTTAGGCCGAT3330.15106288389479128No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA3300.1497019570128562No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTACAGAGTGTTTTAGATCA3250.14743374554296446No Hit
CCCCATTAGAAACCCGAGTAGTCCGGCTGACTGACTGAACTTAGGCCGAT3090.1401754688393108No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA3000.13609268819350567No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGCA2990.1356390458995273No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA2980.13518540360554895No Hit
CCCCATTAGATACCCTAGTAGTCCGGCTGACTGACTGAACTTAGGCCGAT2940.13337083442963554No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2910.13200990754770048No Hit
TACGGAGGGTGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGAGTTCGTA2910.13200990754770048No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA2850.12928805378383038No Hit
CACGATTAACCCAAGTCAATAGAAGCCTGCGTAAAGAGTGTTTTAGATCA2800.12701984231393862No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA2770.12565891543200355No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA2760.1252052731380252No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA2700.12248341937415509No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGAGTGTAAAGGGAGCGCA2630.11930792331630662No Hit
CCCCATTAGATACCCTGGTAGTCCGGCTGACTGACTGAACTTAGGCCGAT2530.1147715003765231No Hit
CCCCATTAGAAACCCGTGTAGTCCGGCTGACTGACTGAACTTAGGCCGAT2460.11159600431867463No Hit
CCCCATTAGATACCCTTGTAGTCCGGCTGACTGACTGAACTTAGGCCGAT2410.10932779284878288No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA2320.1052450122029777No Hit
CACGATTAACCCAAGTCAATAGAAACCGGCATAAAGAGTGTTTTAGATCA2230.10116223155717255No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTTTTTTAGATCA2220.10070858926319419No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)