FastQCFastQC Report
Wed 31 May 2023
EGAF00006172829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28468
Sequences flagged as poor quality0
Sequence length200
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT1016635.71027118167767No Hit
CAGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAAAAGGCAGAGCAGA388513.646901784459745No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGTGTAGCC6552.3008290009835606No Hit
CCCAGTTTGTGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT5722.009273570324575No Hit
CAGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAACAGGCAGAGCAGA4081.4331881410706757No Hit
CCACTACAGGGCTTGGGGCAAATTTTAAAAGGTACCCTTCCCGTGGGTTA3311.162709006603906No Hit
CAGGTAGTTAGACCAGCACGTAAAGCGTCTAATACAAAAGGCAGAGCAGA3191.1205564142194746No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCATATATGTTAAAGCCACTTT2320.8149501194323452No Hit
CCCAGTTTGGGTCTTAGCTCTTGTGTGTTCAGATATGTTAAAGCCACTTT2200.7727975270479135No Hit
CCCAGTTTTGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT2150.7552339468877336No Hit
CAGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAAAAGGCCGAGCAGA2020.7095686384712659No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATCTGTTAAAGCCACTTT1910.6709287621188703No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1830.6428270338625826No Hit
CCGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAAAAGGCAGAGCAGA1570.5514964170296472No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGCTATGTTAAAGCCACTTT1540.5409582689335394No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCCCTTT1500.5269074048053956No Hit
CCCATTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT1390.48826752845299987No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAACGGGCCAGTG1280.4496276521006042No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG1160.4074750597161726No Hit
CCCAGTTTGGGTCTTAGTTATTCTGTGTTCAGATGCATTAAAGCCACTTT1080.3793733314598848No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGACCAGAA950.3337080230434172No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATGCGTTAAAGCCACTTT770.2704791344667697No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAGTATCGTATTAGTA720.25291555430658985No Hit
CCCATTTGCTCCCCTAGCTTTCGTTCCTCAGCGTCAGTTACAGCCCAGCA700.24589012224251794No Hit
CAGGTAGTTAGACCAGCACGTACAGAGTCTAATACAAAAGGCAGAGCAGA690.24237740621048195No Hit
CAGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAAAAGGCAGCGCAGA690.24237740621048195No Hit
CAGGTAGTTAGACCAGCACGTAAAGCGTCTAATACAACAGGCAGAGCAGA660.23183925811437403No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGCGTAGTA630.2213011100182661No Hit
CCCAGTTTGTGCCTTAGCTGTTGTGTCCTCAGGACATTAAAGTCCCTTTC610.2142756779541942No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG580.2037375298580863No Hit
CAGGTAGTTAGACCAGCACGTAAAGAGTCTAATACACAAGGCAGAGCAGA580.2037375298580863No Hit
CCCATTTTGTGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT580.2037375298580863No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGATGGTCCAGTT500.1756358016017985No Hit
CAGGTAGTTAGACCAGCACGTAAAGATTCTAATACAAAAGGCAGAGCAGA490.17212308556976255No Hit
CAGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAACAGGCCGAGCAGA470.1650976535056906No Hit
CCCAGTTTGTGTCTTAGCTATTGTGTGTTCAGATCTGTTAAAGCCACTTT460.16158493747365463No Hit
CCCAGTTTGGTTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT450.15807222144161864No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG450.15807222144161864No Hit
CCGGTAGTTAGACCAGCACGTAAAGAGTCTAATACAACAGGCAGAGCAGA450.15807222144161864No Hit
CCTGTTCGCTACCCACGCTTTCGTTCCTCAGCGTCAGTTACTGCCCAGAG450.15807222144161864No Hit
CCCAGTTTGTGTCTTAGCTATTGTGTGTTCATATATGTTAAAGCCACTTT430.15104678937754673No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG430.15104678937754673No Hit
CAGGTAGTTATACCAGCACGTAAAGAGTCTAATACAAAAGGCAGAGCAGA430.15104678937754673No Hit
CCCAGTTTGGGCCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT420.14753407334551075No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTTTTCAGATATGTTAAAGCCACTTT420.14753407334551075No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA350.12294506112125897No Hit
CCGGTAGTTAGACCAGCACGTAAAGCGTCTAATACAAAAGGCAGAGCAGA340.119432345089223No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACCTT340.119432345089223No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG330.11591962905718702No Hit
CAGGTAGTTAGACCAGCACGTAAAGCGTCTAATACAAAAGGCCGAGCAGA330.11591962905718702No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAGGCCACTTT310.10889419699311507No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA300.1053814809610791No Hit
CCCAGTTTGTGTCTTAGCTATTGTGTGTTCAGCTATGTTAAAGCCACTTT300.1053814809610791No Hit
CCCAGTTTTGGTCTTAGCTATTGTGTGTTCATATATGTTAAAGCCACTTT300.1053814809610791No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATCTGTTAAAGCCCCTTT300.1053814809610791No Hit
CCCAGTTTTGGTCTTAGCTATTGTGTGTTCAGATCTGTTAAAGCCACTTT290.10186876492904315No Hit
CCCAGTTTCGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT290.10186876492904315No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG290.10186876492904315No Hit
CCCAGTTTGGGTCTTAGCTCTTGTGTGTTCAGATATGTTAAAGCCCCTTT290.10186876492904315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)