FastQCFastQC Report
Mon 22 May 2023
EGAF00006172882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006172882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24709976
Sequences flagged as poor quality0
Sequence length35-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGCCTCATCTCGTATG1483400.6003243386395842TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGCCTCATCGCGTATG762910.30874574706183444TruSeq Adapter, Index 25 (97% over 37bp)
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATGT249090.10080543987578135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)