FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006173200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006173200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450712276
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC18866830.4186003134292264Illumina Single End PCR Primer 1 (96% over 31bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC7778940.1725921483443242No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)