FastQCFastQC Report
Fri 10 May 2024
EGAF00006173535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006173535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373102303
Total Bases56.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC15173620.40668792119463276No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT13518660.3623311861465513No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGC7012490.18795086343918924No Hit
GCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCC6987010.18726794082533443No Hit
CCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCC6688240.17926021753878052No Hit
GAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCG4224560.11322792612191407No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCC3919080.10504035939976496No Hit
TCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCT3753670.10060699089279007No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)