FastQCFastQC Report
Sat 4 Feb 2023
EGAF00006173761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006173761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54751178
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA4646250.8486118782686283No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG2937320.5364852606458987No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC1838280.33575168008257283No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT1464480.2674791764297747No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA1440540.26310666776886515No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG1372900.25075259567931124No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG1331750.2432367756543978No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1276200.23309087523194477No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA1270310.23201509929156225No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA1125490.2055645268490844No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCA938120.17134243212082123No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTC886100.1618412666847095No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT842630.1539017114846369No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTC829830.15156386224237953No Hit
GGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGC717710.1310857640359811No Hit
GGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAG679770.12415623276635253No Hit
GAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAG620820.1133893411389249No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT620720.11337107669171978No Hit
TTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGT600680.10971088147181053No Hit
ATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCAC555140.10139325221459163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)