FastQCFastQC Report
Fri 3 Feb 2023
EGAF00006173798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006173798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4154622
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTTCCCAGTCCTTCTTCTGGTACAGACAATATTCTGGGAAAAGCCC265590.6392639330365073No Hit
GAGTGCACAGCTCAATAGAGCCAGCCAGTATATTTCCCTGCTCATCAGAG233580.5622172125406355No Hit
GTAGTAACAGAAGGAGGTGGAGCAGGATCCTGGACCACTCAGTGTTCCAG221350.5327801181431187No Hit
CCTGTCCAATGGTGACAAAGAAGATGGAAGGTTTACAGCACAGCTCAATA191730.4614860268876446No Hit
TGTCTCTTTCCAAAGACCAGCTTTTCAGATCCGCCCTGAAGAAGTGCTGC185360.4461537054393877No Hit
ACCTCCCTGTCACTGTGTCACTCAGAGCACAGAAGTACACCGCTGAGTCT184140.4432172168731595No Hit
AGACTAAGAACTTGAGAATGATACTGCGAAATCTTATTGAAAATGACTCT172710.4157056887485793No Hit
ATACTGATGTAGGCATCTACTTCTGTGCTGGGCGGGCTTATTCAGGAGGA157240.37847005094566966No Hit
CACTTCCCTCATCGCACCCCTCCTAGAGACCCCCAGCCTTACCTACAACG154560.37201940393133237No Hit
TAGTATTTCTTTCCAGAGAGGACAATTAGTAAACCACAAAAACTCACGGG147730.355579881876137No Hit
CATCTGAGGTTCCCAGTCCTTCTTCTGGTACAGACAATATTCTGGGAAAA141010.3394051251834704No Hit
CACTGAATCAGGACTCAGTCCAGGAAAGTATTATACTCATACACCCAGGA135470.3260705787433851No Hit
ATTCTTCCAGCAAGTATATCTCCTTGTTCATCAGAGACTCACAGCCCAGT128780.3099680307859536No Hit
ATACTTTTGCCTGTGTTGCTAGAGTAGCCCCTCAGAGCACAGAAGTATAC124880.3005808952053881No Hit
CATCTACAGGGAAGAGCCTTAAATTTATACTGGAAAATCTAATTGAACGT109670.26397106644118284No Hit
AGTCTGCACTGTGCGCTCATTGCACAGAGGTAGGTGGCTGAATCACTGGG96850.2331138669173754No Hit
CTACTGATAAATCCAGCAAGTATATCTCCTTGTTCATCAGAGACTCACAG87740.21118648098431098No Hit
TATGTATTGCACAGAGGTAGGTGGCTGAATCACTGGGCTGTGAGTCTCTG82820.1993442484057515No Hit
GAGTTACTGCAATCTCTAAACATAAATTGTGAAGATTTCATGGACACTTA74730.1798719594706811No Hit
CTACTCACAGCCCAGTGATTCAGCCACCTACCTCTGTGCAATGAGCGCAC74420.1791258025399182No Hit
ACTGTAAGTGTTGTTCCACTGCCAAAGAGTTTCTTATAATAAGTGGTCCC63140.15197531809151352No Hit
CGGTCTGTGCGCTCATTGCACAGAGGTAGGTGGCTGAATCACTGGGCTGT51270.12340472851681815No Hit
CACTCAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAG41770.10053862902569717No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)