FastQCFastQC Report
Wed 8 May 2024
EGAF00006173799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006173799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4154622
Total Bases627.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTCTGAT254100.6116079874414567No Hit
GAACTCACACAGAGGTAGGTGGCTGAATCACTGAGCTTGGAGTCTCTGAT224940.5414210967929213No Hit
TTTGTCATTGCACAGAGGTAGGTGGCTGAATCACTGGGCTGTGAGTCTCT196420.4727746591627349No Hit
CTCTCCACTTGGAGAAAGGCTCAGTTCAAGTGTCAGACTCAGCGGTGTAC183880.44259140783445516No Hit
GTCTCTCTGCACATTGTGCCCTCCCAGCCTGGAGACTCTGCAGTGTACTT180910.4354427430461784No Hit
GTCTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGA179660.43243404574471517No Hit
GTTGTGTATTCTTCCGATACTTACCTGTGACAACAAGTGTTGTTCCACTG164340.3955594516179811No Hit
CCAGTAGTCCCTTTGCCAAAGGTGAGTCCGTCAGCACCTCCTCCTGAATA153850.37031046386410127No Hit
AACTATGGTCTAAAAAACAATGAAACCAATGAAATGGCCTCTCTGATCAT136460.328453467006144No Hit
GTTGTGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTCTGATG131010.31533554677176406No Hit
CGAGTCTGCAAGTGTCTGGGGAGCACTGTGCGCTCATTGCACAGAGGTAG127470.30681491601402006No Hit
TGCTCAGTTCCTTCAACTTCACCATCACAGCCTCACAAGTCGTGGACTCA125800.3027952964192651No Hit
TATGTTATTACATTATTCCAGTTTAATCATTTCCTGCCTTCCCTCTATTA118350.2848634605025439No Hit
AGACTTATTCCAGTTTAATCATTTCCTGCCTTCCCTCTATTACCTTGGAA101200.24358413352646766No Hit
AACTAAATCCAGCAAGTATATCTCCTTGTTCATCAGAGACTCACAGCCCA98090.23609849464042695No Hit
CGGTATCCTCCTCAGCTTACAAAATGACAGGATTAAGAGATTAAAGTAAA96860.2331379364957871No Hit
ATTCTACAGACTGCAAGTGTCTGGGGAGCACTGTGCGCTCATTGCACAGA86520.2082499924180828No Hit
ATACTAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAA84980.20454327734268002No Hit
CCGTCTTCATGTGGTACAGACAGTATTCCAGAAAAGGCCCTGAGTTGCTG79560.19149756584353522No Hit
TGGTAGTGTCTGGGGAGCACTGTGCGCTCATTGCACAGAGGTAGGTGGCT71090.17111063292882No Hit
ATACTAAGGCCTTATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAA67060.16141059282890236No Hit
CATCTAGAACTTGAGAATGATACTGCGAAATCTTATTGAAAATGACTCTG64260.1546711108736246No Hit
ACTGTAGTATATCTCCTTGTTCATCAGAGACTCACAGCCCAGTGATTCAG53850.12961467974703836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44920.10812054622538465No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)