FastQCFastQC Report
Wed 8 Feb 2023
EGAF00006173815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006173815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066251
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTATTGCAGGAAAGAACCTAAGTTGCTGATGTCCGTATACTCCAGTGG233751.1312759195276856No Hit
AGACTAAGATGGAAGGTTTACAGCACAGGTCGATAAATCCAGCAAGTATA213101.0313364639629938No Hit
GAGTGTTGATAATGTCCATATACTCCAATGGTGACAAAGAAGATGGAAGG194900.9432542319398756No Hit
CTCTCATACTTATGCAAGCACAGGGAAGAGCCTTAAATTTATACTGGAAA136850.6623106292507541No Hit
TTAGTACAAAGTACTGATGATAAATCAGGACTTTGAATCCCCAACACTTA121770.589328208431599No Hit
TACTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTCT86690.41955212604857783No Hit
AGCTACAGACAGTATTCCAGAAAAGGCCCTGAGTTGCTGATGTACACATA70570.3415364348281017No Hit
TTTGTCGAGGTTCCCAGTCCTTCTTCTGGTACAGACAATATTCTGGGAAA57640.27895933262706224No Hit
ACTGTCCATCAAGTCCGTAGAGAAAGAAGACATGGCCGTTTACTACTGTG38330.18550505238714948No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24170.11697513999993224No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)