FastQCFastQC Report
Wed 8 Feb 2023
EGAF00006173856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006173856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2714010
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCCGTAAAGAAACCACTTCTTTCCACTTGGAGAAAGGCTCAGTTCA254480.9376531405558565No Hit
AATGTCCATATACTCCAATGGTGACAAAGAAGATGGAAGGTTTACAGCAC247390.9115294343056952No Hit
GGGTTTGCCACAATAAACATACAGGAAAAGCACAGCTCCCTGCACATCAC181780.6697838254096337No Hit
CCGTATTTCAGTTTGGAGAAGCAAAAAAGAACAGCTCCCTGCACATCACA178650.6582510749776161No Hit
CCGTCACTGTGCGCTCATTGCACAGAGGTAGGTGGCTGAATCACTGGGCT161340.5944709120452761No Hit
GTCTACCGTAAAGAAACCACTTCTTTCCACTTGGAGAAAGGCTCAGTTCA155720.5737635454548804No Hit
TTAGTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTCTCT143680.5294011444320397No Hit
TAGTGATGGAAGGTTTACAGCACAGGTCGATAAATCCAGCAAGTATATCT137260.5057461099995947No Hit
CAGTAAGTGTTGTTCCACTGCCAAAGAGTTTCTTATAATAATTCTCCGTC116930.4308385009635189No Hit
AATGTGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACT113620.41864252526704027No Hit
ACTGTAGATTAATTGCCACAATAAACATACAGGAAAAGCACAGCTCCCTG110970.4088783755402522No Hit
CGCTAGATGGAAGGTTTACAGCACAGCTCAATAAAGCCAGCCAGTATGTT81750.3012148076094045No Hit
TGACTAAATCAGAAGTGTTTGAATTCCCCCTTCCCCCACTCCCTTCAAAC80730.2974565311107918No Hit
CCTGTGCTGTTACATTGAACAAGACAGCCAAACATTTCTCCCTGCACATC73460.2706695995961695No Hit
GGAGTATCCCAGAGACTCTGCCGTCTACATCTGTCCGAAACCAGTGGCTC70780.2607949123253046No Hit
CTGTCTTGAGCCCCCTTCTTACCTAGCACGGTGAGCCGTGTCCCTGGCCC62390.2298812458318134No Hit
CATCTACAGTGACCGAGGTTCCCAGTCCTTCTTCTGGTACAGACAATATT60840.22417013938784308No Hit
TTGTGTAGCCACAACAACATTGCTACAAATGATTATATCACGTGGTACCA60500.22291738055497218No Hit
CTTCTTTCTTCTGGTACAGACAATATTCTGGGAAAAGCCCTGAGTTGATA57750.212784772347928No Hit
TTGTGAGTGAAAAGAAAGAAGGAAGATTCACAATCTCCTTCAATAAAAGT54040.19911496273042473No Hit
ATCTTACTCACAGATAGTAAATGACTTTCAGAAAGGAGATATAGCTGAAG51760.19071410938058445No Hit
ATACTAGAAGATAACTCAAACCCAACCAGGAATGTTCGTGCAGGAAAAGG43210.15921090931868342No Hit
GAGTAAGCCACATACCGTAAAGAAACCACTTCTTTCCACTTGGAGAAAGG41720.15372087796286676No Hit
ATACTCACAAAGAAACCAGGTACTTAGATCCTCTTGGGAGTGGCAGTGTC40190.14808346321494764No Hit
CTGTCGGAGGGCCACGAGGCAGTAGTACACAGCAGTGTCGCTCAGGGAAA38790.14292504449136148No Hit
CAACTATACTCCAGTGGTAATGAAGATGGAAGGTTTACAGCACAGCTCAA37410.13784031746382658No Hit
ATGTTGCACATATGACACCAGTGAGAGTGATTATTATTTATTCTGGTACA35400.13043430201067793No Hit
TCGTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGA30820.11355890361494615No Hit
TGACTTACTTACCTGTGACAACAAGTGTTGTTCCACTGCCAAAGAGTTTC30760.11333782852679244No Hit
TAAGTGGGCCCTGGATTGCCCAGTGTCCACTCCCCTAAGAACAGGGTTCT30310.11167976536563978No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)