FastQCFastQC Report
Fri 3 Feb 2023
EGAF00006173857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006173857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2714010
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTTTTCCATATTCCATGGATCAGAGCACAGAAGTACACCGCTGAGTCT252060.9287364453336576No Hit
GCTCTCAGGTGTCTGGGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTG219910.8102770439313046No Hit
GAGTCTCACAATTAACTCAGTCCCCTTCCCAAAGTTGAGCATCTAGGTGA170310.6275216377242531No Hit
GGGTTGAGTTCCATCTCCAAACATGAGTCTGGCATTGTTATTCCGGTAGA158510.5840435370540271No Hit
GTAGTGGTTTACAGCACAGGTCGATAAATCCAGCAAGTATATCTCCTTGT155870.5743162331752647No Hit
GAACTAAATACCAGCTTCTGGGCTCCCTGAATTATTATCCGGTACACCGC149240.5498874359342818No Hit
GGGTTTTACAGCACAGCTCAATAAAGCCAGCCAGTATGTTTCTCTGCTCA137700.5073673273127218No Hit
ATTCTCAAGTGTCTGGGGAGCACTGTGCGCTCATTGCACAGAGGTAGGTG135120.497861098522113No Hit
TTAGTGGATGTGTTGGAATCAGGACTCAGTCCAGGAAAGTATTATACTCA111320.41016798022114875No Hit
CTCTTTCCCAGTCCTTCTTCTGGTACAGACAATATTCTGGGAAAAGCCCT102260.37678564190994135No Hit
GAGTGGGAGCACTGTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACT75450.2780019233532669No Hit
ACACTTCCTTCCCCAAAGGTCAACCTAGAGCCACTGGTTTCGGACAGATG69730.25692609828261503No Hit
CTCTTGGTCCCAGCGCCCCAGATTAACTGATAGTTGCTATCTCTTGCTGC69220.2550469600333086No Hit
GAGTGAACAATTTGCAGTGGTTTCATCAAAACCCTTGGGGACAGCTCATC67480.24863578247685159No Hit
GCGTTCATTTAAAAAAAAAAGAAAAAGAAAAAGAAAACACCTTTTCAAAA66460.24487750597823885No Hit
TGTCTGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAG57080.21031610053021174No Hit
TTTGTGCCCTTTCCAAAGACAAGAGGTGTGTTTCCTGCTTGCTGCACAGA54130.19944657536265525No Hit
ACTGTCAGACCCTGGGGCAGGGCCCAGAGTTTCTGACTTACTTCCAGAAT53900.19859912085806608No Hit
TATGTGTTCACGGCACAGAGGTAGGTGGCTGAATCACTGGGCTGGGAGTC52230.19244586423778837No Hit
GGGTCCAAAGATGAGCTTGTCGGTGTTATAAGATCGCCCACCCACGAGGC49960.18408185673597371No Hit
GCACTCTCTCTCCCACCTGGAGCCCCCTTCTTACCTAGCACGGTGAGCCG46370.17085419729477783No Hit
GCTCTCACGATTTATTATTCAAGGATACAAGACAAAAGTTACAAACGAAG38190.14071429360982457No Hit
ATACTTGAGTTCCTCTTCCAAAGTATAGCCTCCCCAGGGTTGAGCCTCTG35720.1316133691474976No Hit
TGGTGACATTTCAGTTTGGAGAAGCAAAAAAGAACAGCTCCCTGCACATC34400.1267497172081164No Hit
CTGTCACTTACTTGGTATGACCACCACTTGGTTCCCCTTCCCAAAAGCGA33740.12431789123842579No Hit
GGTGTAAGAGAAAGGACCTCAGAGGACACCACTTAACTTTAAGCAATATC33420.12313882410160612No Hit
TTGTGTCCCTTTTCCAAAGATGATCTTGTCATCTTCTATCCCCCAGCTGT33010.12162814433255588No Hit
TAGTGGATAGGATACCTGAAACGTCTACATCCACTCTCACCATTCACAAT31760.117022413329354No Hit
AAGTAGACAATATTCTGGGAAAAGCCCTGAGTTGATAATGTTCATATACT30300.11164291951761415No Hit
ACCTACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTATC27260.10044178171782712No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)