FastQCFastQC Report
Wed 8 Feb 2023
EGAF00006173860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006173860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251261
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTATATATGCTGCTGTCTTCAGGGCTCATGTTGCTCACAGTCAGAGTT239141.0622491128305425No Hit
TGCTGTCCTGAGGTGACATACATCCTCCTTAGCTGAAAGGATTAAGAGAT102770.4564997128276108No Hit
ACTGTAGTTAACACAATCATCACAGGGTCCTGAGGCAATATACATCCTCC81500.3620193304996622No Hit
CATCTGAAAGAGTACTGTCCACATGGTCCTGAAGCGACATACATCCTCCT77610.34474012564513845No Hit
ACAGTATTTCTTATGCCTGTCTTTACTGCAATCTCTAAACATAAATTGTA74790.33221381261435257No Hit
TACTAAGGCTACGGTGTCTCCCGAGAGGAGAAGGGGCTGTTTCTTCTCAT67180.2984105352511326No Hit
GTTGTTAAACATAAATTGTAAAGATTTCATGGACACTTATCACTTCCCTA65630.29152550503917585No Hit
AAAGTAATCTCTGAACATAAAATGTGAAGATTTCATGGACACTTATCACT60020.2666061376268678No Hit
ACCTGACAGGCATAGGAAATCACAAGGATATTGATTGGGGAAGTGATAAG40230.17869984866259397No Hit
ATTCTTAAAGTAAAGACAGGCATAGGAAATCACAAGGATATTGACTGGGG38440.17074874925652778No Hit
ATCTATTGGGGAAATGCTCAAGCTTGTTTCAAGAAACACAGTACCCCTCT36720.1631085866987435No Hit
GATCTACAGGGCTAGGACACATTTCCGCACAGAGAGACAATGGCTGTGCT36180.16070993101199726No Hit
AGGTCTCTAAACATAAATTGTGAAGATTTCATGGACACTTATCACTTCCC34910.15506864819316818No Hit
ATACTTATCCTGTTCTTTTTTCAAGGTGCTCAGATTTCATATTGCACAAA29790.13232583871883358No Hit
ACTGTAACGCAATCATCACAGGGTCCTGAGGTGACATACATCCTCCTCAG26280.1167345767549831No Hit
TACTGAAGATTTCGTGGACACTTACCAGTTCCCAAATAATACTGTCATAA26220.11646805945645573No Hit
AATGTGACACACATGCTGTACAATTTGTGTAGTTAACGCAATTATTACAA25640.11389172557069126No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)