FastQCFastQC Report
Wed 8 Feb 2023
EGAF00006173861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006173861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251261
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCATTCTCAACTCTGACTGTGAGCAACATGAGCCCTGAAGACAGCAG244761.0872128997926052No Hit
TCGTGTCTCTGAACATAAATTGTGAAGATTTCATGGACACTTATTACTTC102730.4563220346285926No Hit
CGTGTACACTTATCACTTCCCCAATCAATACCCTTGTGATTTCCTATGCC80340.3568666627281333No Hit
CCCTTTATGGACATTTATTAGTTCCCCAAATTAATACTTTTATAATTTCC74180.3295042200793244No Hit
CTTCTATTAAGAGATTAAAGTAAAGACAGGCATAGGAAATCACAAGGGTA72640.32266360941712224No Hit
AAAGTCAATTTGTGCAGTTAATGCAATTATCACAGGGTCCTGAGGCGACA62360.2770003122694348No Hit
AGGTATAGGAAAATCTCAAGAAAATATTAAAAATGAAAAAATTAAACTAG59550.2645184187884035No Hit
TAGTAGGGGCCTGAAGCAACATACATCCTCCCCAGCTTACGAGGATGACG56520.25105929521277187No Hit
GTTGTACTTTTCTAATTTCTTATGCCTGTCTTTACTGCAACCTCTAAACA40460.17972149830694886No Hit
TTAGTTGAAGATTTCATGGACATTTATTAGTTCCCCAAATTAATACTTTT36970.1642190754426075No Hit
TCTCTATCACCCAGATGCCAAGATATCACATTGTACAGAAGAAAGAGATG35430.1573784647804053No Hit
AATGTACCCAAAGCAAAATCTGAGACTGATCTATTACTCAAGGGTTGAAA33540.1489831698767935No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33150.14725080743636568No Hit
TACTAGAGTTAATTTCCTGCACAGTCTAGAAGTCCAGTCTTTGGCATCTC31330.13916644938103578No Hit
GGCTGTCAATACCCCTGTGATTTCCTATGCCTGTCTTTACTTTAATCTCT28270.1255740671561405No Hit
AATGTATCATAGTGGCCCTGAGGTGACATACATCCTCAGCTTACGAAGCT26010.11553524891160998No Hit
TTAGTACTTATCACTTCCGCAATCGATACCCTTGTGATTTCCTATGCCTG25590.11366962782191846No Hit
ATACTATTTCTTATGCCTGTCTTTACTGCAATCTCTAAACATAAATTGTA24060.10687343670947082No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)