FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402269375
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA25037030.6223946329496249TruSeq Adapter, Index 1 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA11535250.2867543670208551TruSeq Adapter, Index 1 (100% over 62bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5031190.12507017219493777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)