FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406081216
Sequences flagged as poor quality0
Sequence length75
%GC30

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA272405536.708153917663603TruSeq Adapter, Index 7 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA164416674.048861742967199TruSeq Adapter, Index 7 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC26158640.6441726179228147TruSeq Adapter, Index 7 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC22708420.5592088258522157TruSeq Adapter, Index 7 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA15444210.3803231814593463TruSeq Adapter, Index 7 (100% over 62bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9533430.23476658422929858TruSeq Adapter, Index 7 (100% over 59bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA8496550.20923277574109708TruSeq Adapter, Index 7 (98% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA7981120.196539994600489TruSeq Adapter, Index 7 (98% over 62bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5698710.14033424289194407TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5357760.1319381391923334TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5031480.12390329327619035TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5017670.12356321352229205TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA4962680.12220905091064344TruSeq Adapter, Index 7 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4756910.11714183795194309TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4739390.1167103971635073TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4655280.11463913662039468TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4417790.10879079912920671TruSeq Adapter, Index 7 (98% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACC4323380.1064658947435776TruSeq Adapter, Index 7 (100% over 62bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4308080.10608912282216963TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG4273800.1052449567132896TruSeq Adapter, Index 7 (100% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4179210.1029156197168204TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4152590.1022600858248021TruSeq Adapter, Index 7 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)