FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267857477
Sequences flagged as poor quality0
Sequence length75
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA151166275.643533706546486TruSeq Adapter, Index 11 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA20726530.77378948805674TruSeq Adapter, Index 11 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC8625130.3220044516435134TruSeq Adapter, Index 11 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA4366850.163028863293594TruSeq Adapter, Index 11 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3289170.1227955268166735TruSeq Adapter, Index 11 (98% over 62bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3287180.1227212335760185TruSeq Adapter, Index 11 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA2972100.11095826158326728TruSeq Adapter, Index 11 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA2823580.10541352183347863TruSeq Adapter, Index 11 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA2708860.101130647176222TruSeq Adapter, Index 11 (98% over 62bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA2703810.10094211407807743TruSeq Adapter, Index 11 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA2688830.10038286144239313TruSeq Adapter, Index 11 (98% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)