FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355588217
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7645219621.500205109439833TruSeq Adapter, Index 18 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA99464952.797194767564528TruSeq Adapter, Index 18 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA23642880.6648949225446353TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA16897730.47520500376985214TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA14792200.41599241180705376TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA14711790.41373108828293936TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA14576450.40992500041136065TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA14312700.4025077130156987TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA13944270.39214657104343814TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA13650140.38387492462946265TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA13587190.3821046185003369TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA13329590.37486028396717097TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA13240980.37236835662639517TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA12897660.3627133685366183TruSeq Adapter, Index 18 (97% over 43bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA12673080.35639763620176423TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA11646840.3275372873224312TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA11355810.3193528204001203TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC10844450.3049721414137859TruSeq Adapter, Index 18 (97% over 39bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA10360260.2913555484882673TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7303110.20538110237775398TruSeq Adapter, Index 18 (97% over 39bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4009520.11275739207072769TruSeq Adapter, Index 18 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)