FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405898169
Sequences flagged as poor quality0
Sequence length75
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA129296693.185446495571652TruSeq Adapter, Index 8 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA128329723.161623525333025TruSeq Adapter, Index 8 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA21798040.5370322328307916TruSeq Adapter, Index 8 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC18394970.45319174622835023TruSeq Adapter, Index 8 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC10109500.24906493234267335TruSeq Adapter, Index 8 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA7538320.18571948768756333TruSeq Adapter, Index 8 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC4251900.10475287460584726TruSeq Adapter, Index 8 (98% over 62bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)