FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397836663
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA159046103.9977738301107757TruSeq Adapter, Index 10 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA44826391.126753619487302TruSeq Adapter, Index 10 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC8978240.22567653600090645TruSeq Adapter, Index 10 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC5573620.14009819904406345TruSeq Adapter, Index 10 (100% over 62bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4164930.10468944638217016TruSeq Adapter, Index 10 (98% over 63bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)