FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403128556
Sequences flagged as poor quality0
Sequence length75
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA196888534.884013475840198TruSeq Adapter, Index 14 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA194161644.816370289580775TruSeq Adapter, Index 14 (97% over 43bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA12308000.30531203549867103TruSeq Adapter, Index 14 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4251300.1054576744000244TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4199450.10417148419523026TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4166300.103349165867575TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4155240.10307481169852925TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTTCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4092900.10152840673484811TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4085280.10133938514640972TruSeq Adapter, Index 14 (97% over 41bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4083930.10130589706971788TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4076900.10113151101109295TruSeq Adapter, Index 14 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)