FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388556934
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA263877336.791214025793193TruSeq Adapter, Index 16 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA159021414.09261542093597TruSeq Adapter, Index 16 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA22536050.5799935100373218TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6527650.16799725931541348TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC6439120.16571882873669164TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5670710.14594283369551192TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5593380.1439526491631211TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5581010.14363429169945016TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5467400.14071039586697995TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5416800.13940814140766306TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5290450.13615636569749132TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5282610.13595459346505961TruSeq Adapter, Index 16 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5176120.1332139397620427TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5028860.12942401897787262TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5001090.12870932320049655TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4926890.12679969314355358TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4513010.1161479722814572TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4362190.11226643043256049TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4319090.11115719787926884TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)