FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240565691
Sequences flagged as poor quality0
Sequence length75
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5456875422.683514749407887TruSeq Adapter, Index 3 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA40291661.6748714179695725TruSeq Adapter, Index 3 (100% over 62bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA14052520.5841448105748379TruSeq Adapter, Index 3 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA13225240.54975586689126TruSeq Adapter, Index 3 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA12591050.5233934210510509TruSeq Adapter, Index 3 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA12539630.5212559591467264TruSeq Adapter, Index 3 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA12426240.5165424856863733TruSeq Adapter, Index 3 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA12187730.506627938062872TruSeq Adapter, Index 3 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA11996950.4986974638873171TruSeq Adapter, Index 3 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA11606350.48246073460242506TruSeq Adapter, Index 3 (98% over 63bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA10908500.45345202612453994TruSeq Adapter, Index 3 (98% over 63bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA10881910.4523467147274962TruSeq Adapter, Index 3 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA10784160.44828337553753667TruSeq Adapter, Index 3 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC9257870.3848375036987298TruSeq Adapter, Index 3 (100% over 63bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3534050.14690581958339188TruSeq Adapter, Index 3 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)