FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211275109
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7268749234.404190983046654TruSeq Adapter, Index 5 (100% over 63bp)
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA24163571.1437016937002267TruSeq Adapter, Index 5 (98% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA23936201.1329398959155195TruSeq Adapter, Index 5 (100% over 62bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA18561350.8785393645211655TruSeq Adapter, Index 5 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA17901010.8472843812377349TruSeq Adapter, Index 5 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA17658100.8357870495761998TruSeq Adapter, Index 5 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA16839700.7970508253293577TruSeq Adapter, Index 5 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA16661080.7885964455945447TruSeq Adapter, Index 5 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA15875690.751422639190308TruSeq Adapter, Index 5 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA15073020.7134309418342318TruSeq Adapter, Index 5 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA15035720.7116654712032358TruSeq Adapter, Index 5 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA15010530.7104731868816595TruSeq Adapter, Index 5 (98% over 63bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA14701180.6958311402409453TruSeq Adapter, Index 5 (98% over 63bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA13719270.6493557175256267TruSeq Adapter, Index 5 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC7586620.3590872600141458TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)