FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211275109
Sequences flagged as poor quality0
Sequence length75
%GC37

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA182229378.625217180694959Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAACAA52335132.4771081765244762Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAACA39191131.8549809386206495Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAC32866781.5556390033621992Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAACAAAA29272431.3855124788978335Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAACACAA17689200.8372590639629016Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAACAC16353410.7740339161297037Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAGA16150360.7644232241290667Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAACAAA13089320.619539143155761Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAACCA12199900.5774414249621804Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAACAACA11789280.5580061018924857Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAACAAAC9673920.45788261787146917Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAACC9038540.42780903262958436Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAACACAC8656410.409722188334074Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGAAAA8162000.38632094611769907Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA7869200.37246223832264125Illumina Single End PCR Primer 1 (98% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAACACCA6713510.31776152106966843Illumina Single End PCR Primer 1 (100% over 57bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAAA6225320.29465468173063397Illumina Single End PCR Primer 1 (100% over 56bp)
GATCGGAAGAGCATCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA5515800.26107192778681754Illumina Single End PCR Primer 1 (98% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAACACA5478030.2592842112792378Illumina Single End PCR Primer 1 (100% over 57bp)
AATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA5028120.23798922759045885Illumina Single End PCR Primer 1 (98% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAACGA5012660.23725748024581544Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAAGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA4762860.22543403349989513Illumina Single End PCR Primer 1 (98% over 57bp)
GATCGGAAGAGCGTCGTGTAGGAAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA4744450.22456265778095044Illumina Single End PCR Primer 1 (98% over 57bp)
GATCGGAAGAGCGTCGTGTAGAGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA4727180.223745240145634Illumina Single End PCR Primer 1 (98% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAACAACC4404220.20845900971704148Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAACCC4404190.2084575897674723Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAACCAA4403180.20840978479864375Illumina Single End PCR Primer 1 (100% over 57bp)
GATCAGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA4279330.2025477596606044Illumina Single End PCR Primer 1 (98% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAG4260760.20166881087729083Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCATGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA4224570.19995587838035384Illumina Single End PCR Primer 1 (98% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAACAAAAA4211120.19931926765684455Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGAAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA4194480.19853166896248056Illumina Single End PCR Primer 1 (98% over 57bp)
GATCGGAAGAGCGTCGTATAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA4113010.19467555924914942Illumina Single End PCR Primer 1 (98% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAACAAC4108940.19448291942426593Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAAAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA4039520.19119715612121635Illumina Single End PCR Primer 1 (98% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAACAAGA4011470.18986950327404634Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGAAGA3919750.18552824412458357Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAACGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA3713200.17575189134087726Illumina Single End PCR Primer 1 (98% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAACACCC3694780.1748800423054095Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGACAA3690670.1746855092144338Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAGC3417790.16176964793330198Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGAACA2947410.1395057853218289Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAGAAA2919680.13819327860339667Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAGAA2700020.12779640785796495Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAACAACAA2451890.11605200497139491Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAACACGA2396030.11340805887360825Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAACCAAA2389140.11308194378922318Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAACAAACA2189690.10364164573688611Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGAAAC2184540.10339788772751266Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAACCAC2156950.10209200744040323Illumina Single End PCR Primer 1 (100% over 57bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)