FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216853754
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA8351635438.51275454516688TruSeq Adapter, Index 7 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA54862372.529924845110129TruSeq Adapter, Index 7 (100% over 62bp)
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA34683341.5993884984808704TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA22240221.0255861192054807TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA19074620.8796075533928733TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA19040260.8780230754040809TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA18498130.8530232776140919TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA18227750.8405549668280126TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA18164130.8376211923912555TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA17654550.8141224062000789TruSeq Adapter, Index 7 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA17392220.8020253133362866TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA17114620.7892240592708393TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA17052170.7863442382463898TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA16780110.7737984558939202TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA15766030.7270351427718424TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC3630900.16743542286106794TruSeq Adapter, Index 7 (100% over 63bp)
GATCGGAAGAGCAGACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2733700.12606191728643074TruSeq Adapter, Index 7 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)