FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184172147
Sequences flagged as poor quality0
Sequence length75
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3264803817.726913939923826TruSeq Adapter, Index 11 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA20218031.0977789165915517TruSeq Adapter, Index 11 (100% over 62bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA8267760.4489147862298635TruSeq Adapter, Index 11 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7912830.42964314251057734TruSeq Adapter, Index 11 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC7645120.4151072854680898TruSeq Adapter, Index 11 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7475360.40588982219987907TruSeq Adapter, Index 11 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7197570.39080665112732815TruSeq Adapter, Index 11 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7195740.3907072875682988TruSeq Adapter, Index 11 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7179020.3897994412803365TruSeq Adapter, Index 11 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7034520.38195352090889184TruSeq Adapter, Index 11 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6941240.37688869424973365TruSeq Adapter, Index 11 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6844830.3716539178967165TruSeq Adapter, Index 11 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6543860.35531214174312686TruSeq Adapter, Index 11 (98% over 63bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6541710.3551954031355241TruSeq Adapter, Index 11 (98% over 63bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6273360.34062479599588963TruSeq Adapter, Index 11 (98% over 63bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC2412770.1310062373329448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)