FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209994118
Sequences flagged as poor quality0
Sequence length75
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2418485911.516922107313501TruSeq Adapter, Index 13 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA16038300.7637499637013643TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5671980.27010185113851615TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5621560.26770083150614726TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5273330.2511179860761624TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5194960.24738597678245444TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5169100.24615451371833189TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5110540.24336586418101486TruSeq Adapter, Index 13 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5097350.2427377513497783TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5040690.24003958053720342TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4753540.22636538800577263TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4706210.22411151535206333TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4705440.22407484765835203TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4530460.2157422333134112TruSeq Adapter, Index 13 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250140.10715252510072686No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)