FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216764510
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA7132103632.90254294856663TruSeq Adapter, Index 15 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA25874831.1936838738038806TruSeq Adapter, Index 15 (97% over 39bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA17285110.7974142077040194TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTTAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA16401480.7566496932546753TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA16396080.7564005749834233TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA16098340.7426649316347957TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA15427750.7117285943164774TruSeq Adapter, Index 15 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA15268120.7043643814201873TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA15113990.6972539000964687TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA14649190.6758112755635136TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA14170230.6537154075637197TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA13877710.6402205785439692TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA13345680.6156764315339259TruSeq Adapter, Index 15 (97% over 40bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3145670.14511923561656842TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)