FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190688679
Sequences flagged as poor quality0
Sequence length75
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4272561522.405952584107TruSeq Adapter, Index 19 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA47140512.4721189662234746TruSeq Adapter, Index 19 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA10099630.5296397275897013TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA10091130.5291939748557385TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9810950.5145009159143632TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9605760.5037404449165018TruSeq Adapter, Index 19 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9380250.49191436267697886TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9213090.48314824185236505TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9057590.4749935888957519TruSeq Adapter, Index 19 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA8880300.4656962356952507TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA8826730.4628869446413229TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA8207740.4304261817241914TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA7898680.4142186123173049TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2403070.12602059087105008TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1945140.10200605564004143TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)