FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335896771
Sequences flagged as poor quality0
Sequence length75
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA126136493.7552159142369366TruSeq Adapter, Index 11 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA89447132.662935095616028TruSeq Adapter, Index 11 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC6637810.19761458201097143TruSeq Adapter, Index 11 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC3877490.11543695369432413TruSeq Adapter, Index 11 (100% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)