FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329140584
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA176342995.357679926824217TruSeq Adapter, Index 6 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA86435162.626086365575629TruSeq Adapter, Index 6 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC6864920.2085710584994283TruSeq Adapter, Index 6 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC6692270.2033255795645061TruSeq Adapter, Index 6 (100% over 63bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4604900.13990678220343683TruSeq Adapter, Index 6 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4360580.13248381427189787TruSeq Adapter, Index 6 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4235500.1286836144156565TruSeq Adapter, Index 6 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3888300.11813493045269678TruSeq Adapter, Index 6 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3873360.11768102106788508TruSeq Adapter, Index 6 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3715150.11287426044063893TruSeq Adapter, Index 6 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3689460.11209374289741188TruSeq Adapter, Index 6 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3685530.11197434103112608TruSeq Adapter, Index 6 (98% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA3654850.11104221653808574TruSeq Adapter, Index 6 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3639700.1105819269008771TruSeq Adapter, Index 6 (98% over 63bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3577750.10869975244377642TruSeq Adapter, Index 6 (98% over 63bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3551330.10789705592793139TruSeq Adapter, Index 6 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3494050.10615676613127721TruSeq Adapter, Index 6 (98% over 63bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)