FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294158140
Sequences flagged as poor quality0
Sequence length75
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3297116911.208654297310963TruSeq Adapter, Index 8 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3156579910.730894273400015TruSeq Adapter, Index 8 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC8332890.2832792592447042TruSeq Adapter, Index 8 (100% over 62bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7022810.23874267086404613TruSeq Adapter, Index 8 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6850990.2329015950400013TruSeq Adapter, Index 8 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6790480.23084453824735227TruSeq Adapter, Index 8 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6740030.22912947437048656TruSeq Adapter, Index 8 (98% over 63bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6666900.22664339664372365TruSeq Adapter, Index 8 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6553470.22278730753464787TruSeq Adapter, Index 8 (98% over 62bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6490030.22063064445539396TruSeq Adapter, Index 8 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6466910.2198446726648462TruSeq Adapter, Index 8 (98% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6380160.21689557868430903TruSeq Adapter, Index 8 (98% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6112370.20779197203245844TruSeq Adapter, Index 8 (98% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6074730.20651238820044213TruSeq Adapter, Index 8 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5958380.20255703275795803TruSeq Adapter, Index 8 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5948830.20223237745520148TruSeq Adapter, Index 8 (98% over 63bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5920250.20126079121930812TruSeq Adapter, Index 8 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5854040.19900996110459496TruSeq Adapter, Index 8 (98% over 63bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA5807760.19743665771071303TruSeq Adapter, Index 8 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA5765340.1959945762507201TruSeq Adapter, Index 8 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA5665860.19261272185090644TruSeq Adapter, Index 8 (98% over 62bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA5620040.1910550563040683TruSeq Adapter, Index 8 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA5598210.1903129384758824TruSeq Adapter, Index 8 (98% over 62bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA5582780.18978839069352288TruSeq Adapter, Index 8 (98% over 62bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5379870.1828904003812371TruSeq Adapter, Index 8 (98% over 63bp)
GAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA4247980.14441143801086043TruSeq Adapter, Index 8 (100% over 58bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3948680.13423663883651155TruSeq Adapter, Index 8 (98% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA3430260.1166127852181823TruSeq Adapter, Index 8 (100% over 62bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3362430.11430688268561937TruSeq Adapter, Index 8 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3188380.10838999729873189TruSeq Adapter, Index 8 (98% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC3131490.10645600356325342TruSeq Adapter, Index 8 (100% over 63bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)