FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224835116
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA149162886.634323083232248TruSeq Adapter, Index 10 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA77175603.4325420945365135TruSeq Adapter, Index 10 (100% over 62bp)
GAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA5000560.22241009718428503TruSeq Adapter, Index 10 (100% over 58bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3667190.163105749014758TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3253060.14468647326425646TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3239800.14409670774026243TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3215820.1430301483688162TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3169220.14095751839761544TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3123610.1389289206940432TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3054360.13584888581194762TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2977180.13241614801844387TruSeq Adapter, Index 10 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2771260.12325743635171295TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2727860.1213271329021397TruSeq Adapter, Index 10 (98% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC2579320.11472051367634227TruSeq Adapter, Index 10 (100% over 62bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2549040.11337374896544185TruSeq Adapter, Index 10 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)