FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294708913
Sequences flagged as poor quality0
Sequence length75
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3595528912.200272001953332TruSeq Adapter, Index 12 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3166409110.744191846006368TruSeq Adapter, Index 12 (100% over 63bp)
GAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA18968950.6436503669639608TruSeq Adapter, Index 12 (100% over 58bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC8143260.27631536206711194TruSeq Adapter, Index 12 (100% over 62bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA7804970.2648365779151036TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA7759700.26330048592727834TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA7284000.24715913495293573TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA7255980.24620836628717774TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA7254730.2461659515536946TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA7116310.24146911362670598TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA7113220.24136426440553566TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA7007610.23778072840301237TruSeq Adapter, Index 12 (98% over 62bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6971370.23655104044986927TruSeq Adapter, Index 12 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6696720.22723167520895443TruSeq Adapter, Index 12 (98% over 63bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6678760.2266222603182687TruSeq Adapter, Index 12 (98% over 62bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6588750.22356806018961495TruSeq Adapter, Index 12 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6511880.22095972373933595TruSeq Adapter, Index 12 (98% over 63bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6509930.22089355675510228TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6437350.2184307876701374TruSeq Adapter, Index 12 (98% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6379280.2164603688114448TruSeq Adapter, Index 12 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6257580.21233087035952658TruSeq Adapter, Index 12 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6087630.2065641631951593TruSeq Adapter, Index 12 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5968000.20250490354188916TruSeq Adapter, Index 12 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5755650.19529948861777385TruSeq Adapter, Index 12 (98% over 63bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5670970.19242614491269222TruSeq Adapter, Index 12 (98% over 63bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5280490.17917646080829597TruSeq Adapter, Index 12 (98% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4251870.14427354628395647TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA3043920.10328564443519223TruSeq Adapter, Index 12 (100% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)