FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298424139
Sequences flagged as poor quality0
Sequence length75
%GC30

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3545232011.879843272329923TruSeq Adapter, Index 15 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA122655674.110112218502539TruSeq Adapter, Index 15 (97% over 39bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA7366260.24683861113527417TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTTAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA7135800.23911604550193574TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA7004010.2346998477894578TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6859360.2298527197895342TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6850650.22956085331957682TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6450120.2161393519175069TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6224960.2085943858583102TruSeq Adapter, Index 15 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6157010.2063174252803993TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6056890.20296246879680202TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6047810.20265820386600833TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5620660.18834468347079658TruSeq Adapter, Index 15 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3282110.10998138458229749TruSeq Adapter, Index 15 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)