FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248494484
Sequences flagged as poor quality0
Sequence length75
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4561866518.358019166332884TruSeq Adapter, Index 19 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3192165912.84602317369749TruSeq Adapter, Index 19 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9385740.3777041586162532TruSeq Adapter, Index 19 (97% over 39bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9033800.3635412687872782TruSeq Adapter, Index 19 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8510730.34249170697889614TruSeq Adapter, Index 19 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8390240.3376429071962821TruSeq Adapter, Index 19 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8314950.3346130612702051TruSeq Adapter, Index 19 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8153810.3281284102869664TruSeq Adapter, Index 19 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8127710.32707808516184206TruSeq Adapter, Index 19 (97% over 39bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8083380.3252941421428091TruSeq Adapter, Index 19 (97% over 39bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7634050.3072120506304679TruSeq Adapter, Index 19 (97% over 39bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7498190.3017447260519473TruSeq Adapter, Index 19 (97% over 39bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7348350.29571481353284285TruSeq Adapter, Index 19 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7167850.288451070809282TruSeq Adapter, Index 19 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6583540.26493706797934397TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6411180.2580008979193277TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6011110.24190114417187628TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5983540.24079166280407252TruSeq Adapter, Index 19 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5896920.23730587114360255TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5882530.2367267838428156TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5658200.2276992192712012TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5614450.22593861681050434TruSeq Adapter, Index 19 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5381540.21656577294488355TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5173950.20821186517765924TruSeq Adapter, Index 19 (97% over 40bp)
GAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA5162110.20773539584886722TruSeq Adapter, Index 19 (97% over 35bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4950740.19922937202903868TruSeq Adapter, Index 19 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2881030.11593939445352033TruSeq Adapter, Index 19 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)