FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209522528
Sequences flagged as poor quality0
Sequence length75
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4783359022.829807589950423TruSeq Adapter, Index 14 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA206587809.859932579660361TruSeq Adapter, Index 14 (97% over 44bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9693710.4626571706885858TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9641740.4601767691539117TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9559650.45625881337208757TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9533580.4550145557617556TruSeq Adapter, Index 14 (97% over 40bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9253710.4416570422441638TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTTCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9182170.4382426122693594TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9125580.4355417093860189TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8965860.4279186627606937TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8819420.42092943819387285TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8245140.39352045236873046TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8191210.3909465048073494TruSeq Adapter, Index 14 (97% over 40bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8082390.38575279122252665TruSeq Adapter, Index 14 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4233340.20204700852024848TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4152990.19821209870090917TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4143220.19774580039431366TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4092460.19532314921286176TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3990400.19045207396504876TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTTCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3985440.19021534524440256TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3854760.18397830709640922TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3731780.17810877119619328TruSeq Adapter, Index 14 (97% over 41bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3715460.1773298573411638TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3562240.17001703988604033TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3463240.16529201098604537TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3309010.157930988690629TruSeq Adapter, Index 14 (97% over 41bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA3229360.15412948816654215TruSeq Adapter, Index 14 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2604150.12428973747395794TruSeq Adapter, Index 14 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)