FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263408070
Sequences flagged as poor quality0
Sequence length75
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3344287812.696223771731823TruSeq Adapter, Index 18 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA94431263.5849797616299304TruSeq Adapter, Index 18 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6992840.2654755414289319TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6978240.26492126835749563TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6884190.26135076271581203TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6883940.26134127173856136TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6551970.24873839286700666TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6495680.24660140442925688TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6428150.2440377016543191TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6428010.24403238670705876TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6390130.24259431383404467TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6330880.24034495222564745TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6193070.2351131459260151TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6160610.23388083743979443TruSeq Adapter, Index 18 (97% over 43bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5854080.22224376041326296TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5777080.21932053942007168TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5597750.2125124716186562TruSeq Adapter, Index 18 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)