FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006176842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006176842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280899018
Sequences flagged as poor quality0
Sequence length75
%GC30

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA198572427.0691745885704735TruSeq Adapter, Index 20 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA189780846.756194498337477TruSeq Adapter, Index 20 (97% over 43bp)
GAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA5969080.21249914088343308TruSeq Adapter, Index 20 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4766580.16969016246258292TruSeq Adapter, Index 20 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4326890.154037206352925TruSeq Adapter, Index 20 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4276080.1522283712647226TruSeq Adapter, Index 20 (97% over 41bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4082380.14533265474071538TruSeq Adapter, Index 20 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4064380.1446918550637297TruSeq Adapter, Index 20 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3908610.13914644585905955TruSeq Adapter, Index 20 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3907790.13911725387377466TruSeq Adapter, Index 20 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3894200.13863345011765046TruSeq Adapter, Index 20 (97% over 41bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3827830.13627067930867598TruSeq Adapter, Index 20 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3793500.13504853192473604TruSeq Adapter, Index 20 (97% over 41bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3789410.13490292799813206TruSeq Adapter, Index 20 (97% over 41bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3746690.13338209676475266TruSeq Adapter, Index 20 (97% over 40bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3740540.13316315687511587TruSeq Adapter, Index 20 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3723520.13255724518054385TruSeq Adapter, Index 20 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3653590.1300677384354544TruSeq Adapter, Index 20 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3628590.12917773888408537TruSeq Adapter, Index 20 (97% over 41bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3578540.1273959597822446TruSeq Adapter, Index 20 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3571710.12715281190481056TruSeq Adapter, Index 20 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3549800.12637281629799074TruSeq Adapter, Index 20 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3459720.12316596991449788TruSeq Adapter, Index 20 (97% over 41bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3431040.12214496242916734TruSeq Adapter, Index 20 (97% over 41bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3258430.11600004952669503TruSeq Adapter, Index 20 (97% over 40bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3254990.11587758558842666TruSeq Adapter, Index 20 (97% over 40bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3214140.11442332632148966TruSeq Adapter, Index 20 (97% over 40bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3179480.11318943094347166TruSeq Adapter, Index 20 (97% over 41bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)