FastQCFastQC Report
Tue 28 Jun 2022
EGAF00006184200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006184200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394427952
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTCAAGATGCTACCGTTCAGAGCTCCCTGGTGTTCAATACCCGATGT43792221.1102717182680806No Hit
GTTGTCAAGATGCTACCGTTCAGAGTTACCCGCAGGAAGACGTATACCCC24624640.624312751546574No Hit
GTTGTCAAGATGCTACCGTTCAGAGCCCCACCAGGTTGCTTTGTCGGACG20679870.5243003163224091No Hit
GTTGTCAAGATGCTACCGTTCAGAGATTGGGAGGCTTTCGTACCGCTGCC11482480.29111729890786237No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7758910.19671298549348246No Hit
GTTGTCAAGATGCTACCGTTCAGAGTGTCTACGTCGGACCGCAAGAAGTG6965010.17658510165628424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG6844750.1735361291027366Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC5092990.1291234552261144Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)