FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006184350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006184350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2062399
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAATGCTGCAATGACAACCTCTTATAGGGTTAAAGTCTCACCTTTTGG40560.19666417603965092No Hit
ATAGGTAAGACTAGCAATGTTGTATCCAGGAATGCTGCAATGACAACCTC36610.17751172299831408No Hit
CTAGCAATGTTGTATCCAGGAATGCTGCAATGACAACCTCTTATAGGGTT34740.16844461231798502No Hit
CAGGAATGCTGCAATGACAACCTCTTATAGGGTTAAAGTCTCACCTTTTG34300.16631117451084879No Hit
AAGACTAGCAATGTTGTATCCAGGAATGCTGCAATGACAACCTCTTATAG33420.16204429889657626No Hit
AGACTAGCAATGTTGTATCCAGGAATGCTGCAATGACAACCTCTTATAGG33170.16083211832433977No Hit
AATGTTGTATCCAGGAATGCTGCAATGACAACCTCTTATAGGGTTAAAGT30730.14900123593931144No Hit
AAGATAAAGTCCTCTTATCATTTTGCCTTTGTGTCCTATAGTGGAACACC30720.148952748716422No Hit
AGCAATGTTGTATCCAGGAATGCTGCAATGACAACCTCTTATAGGGTTAA29480.14294033307812892No Hit
CAACAAGATAAAGTCCTCTTATCATTTTGCCTTTGTGTCCTATAGTGGAA29060.14090386971677157No Hit
CACATAGGTAAGACTAGCAATGTTGTATCCAGGAATGCTGCAATGACAAC28490.13814009801207236No Hit
CAATGTTGTATCCAGGAATGCTGCAATGACAACCTCTTATAGGGTTAAAG27930.1354248135302626No Hit
ATCCAGGAATGCTGCAATGACAACCTCTTATAGGGTTAAAGTCTCACCTT27790.13474599240981014No Hit
AACAAGATAAAGTCCTCTTATCATTTTGCCTTTGTGTCCTATAGTGGAAC26800.12994575734375358No Hit
AGGTAAGACTAGCAATGTTGTATCCAGGAATGCTGCAATGACAACCTCTT26230.12718198563905433No Hit
CCCACATAGGTAAGACTAGCAATGTTGTATCCAGGAATGCTGCAATGACA25990.1260182922897073No Hit
TAAGACTAGCAATGTTGTATCCAGGAATGCTGCAATGACAACCTCTTATA25210.12223628890432937No Hit
AAAAGGTGAGACTTTAACCCTATAAGAGGTTGTCATTGCAGCATTCCTGG24920.12083015944053502No Hit
AATGCTGCAATGACAACCTCTTATAGGGTTAAAGTCTCACCTTTTGGTGT24900.1207331849947561No Hit
CTATAAGAGGTTGTCATTGCAGCATTCCTGGATACAACATTGCTAGTCTT24860.12053923610319828No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24600.1192785683080723No Hit
AAGGTGAGACTTTAACCCTATAAGAGGTTGTCATTGCAGCATTCCTGGAT24550.11903613219362501No Hit
ATACTTCTTTTGCCAAAAGAAGTATAGCCAACAAGATAAAGTCCTCTTAT24190.11729059216960443No Hit
ATGTTGTATCCAGGAATGCTGCAATGACAACCTCTTATAGGGTTAAAGTC24170.1171936177238255No Hit
CTATACTTCTTTTGCCAAAAGAAGTATAGCCAACAAGATAAAGTCCTCTT23970.11622387326603631No Hit
ATACTTCTTTTGGCAAAAGAAGTATAGCCAACAAGATAAAGTCCTCTTAA23750.11515715436246818No Hit
ACATAGGTAAGACTAGCAATGTTGTATCCAGGAATGCTGCAATGACAACC23660.11472076935646304No Hit
CATAGGTAAGACTAGCAATGTTGTATCCAGGAATGCTGCAATGACAACCT23480.11384799934445275No Hit
ACTAGCAATGTTGTATCCAGGAATGCTGCAATGACAACCTCTTATAGGGT23090.1119569976517638No Hit
CTATACTTCTTTTGGCAAAAGAAGTATAGCCAACAAGATAAAGTCCTCTT23010.1115690998686481No Hit
GTAAGACTAGCAATGTTGTATCCAGGAATGCTGCAATGACAACCTCTTAT22920.11113271486264298No Hit
AAAGGCAAAATGATAAGAGGACTTTATCTTGTTGGCTATACTTCTTTTGG22840.1107448170795273No Hit
CAAGATAAAGTCCTCTTATCATTTTGCCTTTGTGTCCTATAGTGGAACAC22350.10836894315794374No Hit
CTATAGGACACAAAGGCAAAATGATAAGAGGACTTTATCTTGTTGGCTAT22270.10798104537482806No Hit
AAAAGAAGTATAGCCAACAAGATAAAGTCCTCTTATCATTTTGCCTTTGT22170.10749617314593345No Hit
CAAAAGAAGTATAGCCAACAAGATAAAGTCCTCTTATCATTTTGCCTTTG21900.10618701812791802No Hit
CTATGTGGGCTACCCCACATAGGTAAGACTAGCAATGTTGTATCCAGGAA21880.10609004368213909No Hit
AAGGCAAAATGATAAGAGGACTTTATCTTGTTGGCTATACTTCTTTTGGC21860.10599306923636018No Hit
AAAAGAAGTATAGCCAACAAGATAAAGTCCTCTTAACACCAAAAGGTGAG21820.10579912034480235No Hit
CTATGTGGGGTAGCCCACATAGGTAAGACTAGCAATGTTGTATCCAGGAA21790.10565365867613397No Hit
AATGACAACCTCTTATAGGGTTAAAGTCTCACCTTTTGGTGTTAAGAGGA21560.10453845254967636No Hit
CTCTTATCATTTTGCCTTTGTGTCCTATAGTGGAACACCAAAAGGTGAGA21300.10327778475455039No Hit
AATGACAACCTCTTATAGGGTTAAAGTCTCACCTTTTGGTGTTCCACTAT21250.10303534864010311No Hit
CTATAGTGGAACACCAAAAGGTGAGACTTTAACCCTATAAGAGGTTGTCA20760.10065947471851955No Hit
CACAAAGGCAAAATGATAAGAGGACTTTATCTTGTTGGCTATACTTCTTT20690.10032006415829334No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)